Anti-STAT6 抗体 [YE361] (ab32520)

Thank you for contacting us.



The antibody is from tissue culture supernatant.

The Jurkat lysate will serve as a positive control for STAT6, in addition to IRF7. Although ab32520 has not been tested on Jurkat, other STAT6 antibodies have been, and the results demonstrate that Jurkat expresses this protein. For an example western blot of Jurkat stained for STAT6, please see the datasheet for ab125303: https://www.abcam.com/stat6-antibody-ab125303.html.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1180962.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for taking the time to complete our questionnaire and contact us. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details.

I can recommend you may like to consider trying the following options:

1. The time for antigen retrieval can sometimes require some optimization. For example I can recommend to try , 5, 10 and 20 minutes if this has not already been done.

2. We recommend not to mix blocking agents in one experiment. I can recommend to try serum only (no casein).

3. I can suggest to try overnight incubation at 4oC. This sometimes provides more specific and efficient staining.

4. Could you confirm if the current vial of secondary antibody is working well with other primary antibodies?

Alternatively, or if these tips do not work, I am pleased to offer you a free of charge replacement or credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch.

I would also like to reassure you that this antibody is tested and covered by our 6 month guarantee for IHC-P and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:
Lot number:
Purchase order number
or preferably Abcam order number:
General Information
Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, low signal, non-specific satining etc.)
Sample (Species/Tissue/Cell Type/Cell Line etc.)
Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
Permeabilization step
Blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method
Positive and negative controls used (please specify)
Optimization attempts (problem solving)
How many times have you tried the IHC?
Have you run a "No Primary" control?
Yes No
Do you obtain the same results every time?
Yes No
What steps have you altered?
Additional Notes
We would appreciate if you are also able to provide and image which woudl help us to assess the results

Thank you for contacting Abcam regarding ab32520.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for contacting us regarding ab32520. I'm sorry to hear you are experiencing problems with this antibody which has been tested in IHC-P and should work in lung tissue of mouse and human. Could you please confirm the species of your samples? If it is not from the above species then the problem may be that the antibody does not recognise the species you are working with. I would like to recommend trying the following steps to improve your signal: 1)reduce the fixation time as overfixed tissue shows modified antigens which cannot be recognized by the antibody 2) vary the antigen retrieval time as too little or too much can also modify the antigen such that it is not optimally recognized by the antibody 3) use PBS containing 0.3% triton to dilute the blocking buffer and antibodies in 4) check that the tissue is washed in PBS a lot before adding the antibody, as the remaining fixative can fix the antibody and prevent its binding 5) check that the secondary antibody works with other primary antibodies, as well as your ABC kit. As you have tried this staining once I would first recommend to run this again as it may be that there was a problem in one of the steps in your first attempt. I can also please make sure that endogenous peroxidases are blocked? I hope this information helps, please do not hesitate to contact us if you need any more advice or information or still experience a problem following the modifications advised. I can offer you a refund or replacement vial if the antibody was purchased in the last 90 days,

Thank you for your reply. For ab32520, HIER using Citrate buffer pH 6 was used as the pretreatment on FFPE tissue. You may find a protocol for this at the following link: https://www.abcam.com/assets/pdf/protocols/IHC-paraffin%20protocol%20(IHC-P).pdf I hope this information helps. Please do not hesitate to contact us if you need anything further.