Anti-STAT6 抗体 [EPR18278-222] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (AB251555)

This data was developed using ab217998, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed Raji (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Raji cell line [PMID : 11781246] [PMID : 23575898].

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (AB251555)

This data was developed using ab217998, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed Daudi (Human Burkitt's lymphoma cell line) cells labeling STAT6 with ab217998 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on Daudi cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (AB251555)

This data was developed using ab217998, the same antibody clone in a different buffer formulation.

STAT6 was immunoprecipitated from 0.35 mg of Raji (Human Burkitt's lymphoma cell line) whole cell lysate with ab217998 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab217998 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Raji whole cell lysate. 10 μg (Input).

Lane 2 : ab217998 IP in Raji whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab217998 in Raji whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), respectively which is consistent with the Uniprot annotation.

All lanes:

Immunoprecipitation - Anti-STAT6 antibody [EPR18278-222] (<a href='/products/primary-antibodies/stat6-antibody-epr18278-222-ab217998'>ab217998</a>)

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false

• WB

Supplier Data

Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (AB251555)

This data was developed using ab217998, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-3 : 8 seconds; Lanes 4-5 : 2 seconds; Lanes 6-7 : 1 second.

The molecular weight observed is consistent with what has been described in the literature (PMID : 21411736). This antibody recognizes three isoforms of STAT6, isoform 1 (110kDa), isoform 2 (75kDa), isoform 3 (81kDa), which is consistent with the Uniprot annotation.

All lanes:

Western blot - Anti-STAT6 antibody [EPR18278-222] (<a href='/products/primary-antibodies/stat6-antibody-epr18278-222-ab217998'>ab217998</a>) at 1/1000 dilution

Lane 1:

Human thymus lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

P388D1 (Mouse lymphoma N106 O91monocyte; macrophage cell line) whole cell lysate at 20 µg

Lane 4:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 5:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 6:

Daudi (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 7:

Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false

• WB

Supplier Data

Western blot - Anti-STAT6 antibody [EPR18278-222] - BSA and Azide free (AB251555)

This data was developed using ab217998, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 10 seconds; Lane 2 : 3 seconds; Lane 3 : 5 seconds.

All lanes:

Western blot - Anti-STAT6 antibody [EPR18278-222] (<a href='/products/primary-antibodies/stat6-antibody-epr18278-222-ab217998'>ab217998</a>) at 1/2000 dilution

Lane 1:

Mouse spleen tissue lysate at 10 µg

Lane 2:

Rat spleen tissue lysate at 10 µg

Lane 3:

A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa,75 kDa,81 kDa

false