Anti-STAT5b (phospho S731) 抗体 (ab52211)
Key features and details
- Rabbit polyclonal to STAT5b (phospho S731)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-STAT5b (phospho S731) antibody
STAT5b 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STAT5b (phospho S731) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human STAT5b aa 650-750 (phospho S731).
Database link: P51692 -
特記事項
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52211の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. -
関連疾患
Growth hormone insensitivity with immunodeficiency -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 6777 Human
- Entrez Gene: 20851 Mouse
- Omim: 604260 Human
- SwissProt: P51692 Human
- SwissProt: P42232 Mouse
- Unigene: 595276 Human
- Unigene: 34064 Mouse
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別名
- Signal transducer and activator of transcription 5B antibody
- STA5B_HUMAN antibody
- STAT5 antibody
see all
画像
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Western blot - Anti-STAT5b (phospho S731) antibody (ab52211)All lanes : Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution
Lane 1 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min)
Lane 2 : Extracts from RAW264.7 cells
treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide
Predicted band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5b (phospho S731) antibody (ab52211)Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-STAT5b (phospho S731) antibody (ab52211)ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab52211 は 8 報の論文で使用されています。
- Dubois N et al. STAT5b is a marker of poor prognosis, rather than a therapeutic target in glioblastomas. Int J Oncol 61:N/A (2022). PubMed: 36069226
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Lee HY et al. Deletion of Jazf1 gene causes early growth retardation and insulin resistance in mice. Proc Natl Acad Sci U S A 119:e2213628119 (2022). PubMed: 36442127
- Ding N et al. The Role of MiR-5094 as a Proliferation Suppressor during Cellular Radiation Response via Downregulating STAT5b. J Cancer 11:2222-2233 (2020). PubMed: 32127949
- Xiang Y et al. MKL-1 is a coactivator for STAT5b, the regulator of Treg cell development and function. Cell Commun Signal 18:107 (2020). PubMed: 32646440
- Chen W et al. HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway. Cancer Manag Res 11:6625-6635 (2019). PubMed: 31406476
- Agarwal AK et al. Metabolic, Reproductive, and Neurologic Abnormalities in Agpat1-Null Mice. Endocrinology 158:3954-3973 (2017). PubMed: 28973305
- Chen AY et al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB ; Human . PubMed: 21698228