Anti-STAT5a (phospho Y694) 抗体 (ab30648)
Key features and details
- Rabbit polyclonal to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-STAT5a (phospho Y694) antibody
STAT5a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STAT5a (phospho Y694) -
由来種
Rabbit -
特異性
ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human STAT5a (phospho Y694).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30648の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 91 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 6776 Human
- Omim: 601511 Human
- SwissProt: P42229 Human
- Unigene: 437058 Human
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別名
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5a (phospho Y694) antibody (ab30648)Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue. Left: Using ab30648 (10ug/ml); right: the same antibody preincubated with synthesized phosphopeptide.
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Western blot - Anti-STAT5a (phospho Y694) antibody (ab30648)All lanes : Anti-STAT5a (phospho Y694) antibody (ab30648)
Lane 1 : HeLa cells untreated
Lane 2 : HeLa cells treated with EGF
Predicted band size: 91 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab30648 は 9 報の論文で使用されています。
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Jiang Y et al. Loss of STAT5A promotes glucose metabolism and tumor growth through miRNA-23a-AKT signaling in hepatocellular carcinoma. Mol Oncol 15:710-724 (2021). PubMed: 33155364
- Kaihoko Y et al. Distinct expression patterns of aquaporin 3 and 5 in ductal and alveolar epithelial cells in mouse mammary glands before and after parturition. Cell Tissue Res 380:513-526 (2020). PubMed: 31953689
- Wang S et al. Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. J Hematol Oncol 11:82 (2018). PubMed: 29898759
- Matsunaga K et al. IL-1ß directly inhibits milk lipid production in lactating mammary epithelial cells concurrently with enlargement of cytoplasmic lipid droplets. Exp Cell Res 370:365-372 (2018). PubMed: 29966663
- Kobayashi K et al. Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with ß-casein expression in mammary epithelial cells. Biochim Biophys Acta 1863:2006-16 (2016). PubMed: 27130254
- Mace TA et al. Single agent BMS-911543 Jak2 inhibitor has distinct inhibitory effects on STAT5 signaling in genetically engineered mice with pancreatic cancer. Oncotarget 6:44509-22 (2015). IHC . PubMed: 26575024
- Surana R et al. IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model. Cancer Immunol Res 2:1103-12 (2014). PubMed: 25204776
- Bertucci PY et al. Glucocorticoid-induced impairment of mammary gland involution is associated with STAT5 and STAT3 signaling modulation. Endocrinology 151:5730-40 (2010). PubMed: 20881248