Anti-STAT5 (phospho Y694) 抗体 [E208] (ab32364)
![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-250241-anti-stat5-phospho-y694-antibody-e208-western-blot.jpg "Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E208] to STAT5 (phospho Y694)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-STAT5 (phospho Y694) antibody [E208]
STAT5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E208] to STAT5 (phospho Y694) -
由来種
Rabbit -
特異性
This reagent is not recommended for mouse IHC-P
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アプリケーション
適用あり: WB, IHC-P, ELISAmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E208 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32364の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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| IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Unsuitable for mouse. |
| ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
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IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Unsuitable for mouse. |
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ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
see all -
別名
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
画像
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Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (purified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Epidermal growth factor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-250259-anti-stat5-phospho-y694-antibody-e208-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-261378-anti-stat5-phospho-y694-antibody-e208-elisa.jpg)
ELISA - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
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![Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-44577-anti-stat5-phospho-y694-antibody-e208-western-blot.gif)
Western blot - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (unpurified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lane 3 : A431 cell lysate, treated with phosphatase
Predicted band size: 90 kDa
Observed band size: 90 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-44576-anti-stat5-phospho-y694-antibody-e208-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.
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![Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)](https://www.abcam.co.jp/ps/products/32/ab32364/Images/ab32364-6-benefits-of-recombinant-antibodies.png)
Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (70)
ab32364 は 70 報の論文で使用されています。
- Li Y et al. CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice. Pathogens 12:N/A (2023). PubMed: 38003764
- Yang C et al. Methionine orchestrates the metabolism vulnerability in cisplatin resistant bladder cancer microenvironment. Cell Death Dis 14:525 (2023). PubMed: 37582769
- Li J et al. Targeting of STAT5 using the small molecule topotecan hydrochloride suppresses acute myeloid leukemia progression. Oncol Rep 50:N/A (2023). PubMed: 37830151
- Cao L et al. CD27-Expressing Xenoantigen-Expanded Human Regulatory T Cells Are Efficient in Suppressing Xenogeneic Immune Response. Cell Transplant 32:9636897221149444 (2023). PubMed: 36644879
- Vaparanta K et al. STAT5b is a key effector of NRG-1/ERBB4-mediated myocardial growth. EMBO Rep 24:e56689 (2023). PubMed: 37009825