AB28815

Anti-STAT4 (phospho Y693) 抗体

Name: Anti-STAT4 (phospho Y693) 抗体 (ab28815) | Abcam
Brand: Abcam Limited
SKU: AB28815
Price: 59000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-STAT4 (phospho Y693) antibody

(1 Review)

(12 Publications)

Rabbit Polyclonal STAT4 phospho Y693 antibody. Suitable for IP, ELISA, WB, IHC-P and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human STAT4 phospho Y693.

別名を表示する

Signal transducer and activator of transcription 4, STAT4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 (phospho Y693) antibody (AB28815)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 (phospho Y693) antibody (AB28815)

ab28815, staining human STAT4 in breast carcinoma by immunohistochemistry, paraffin embedded tissue. left : Using STAT4 antibody (ab28815); right : The same antibody preincubated with synthesized phosphopeptide.

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Western blot - Anti-STAT4 (phospho Y693) antibody (AB28815)

All lanes:

Western blot - Anti-STAT4 (phospho Y693) antibody (ab28815)

Lane 1:

HeLa cell lysate (cells treated with IL4)

Lane 2:

HeLa cell lysate (untreated)

Predicted band size: 85 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

IHC-P, WB, IP, ELISA

applications

免疫原

Synthetic Peptide within Human STAT4 phospho Y693. The exact immunogen used to generate this antibody is proprietary information.

Q14765

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Signal Transducer and Activator of Transcription 4 (STAT4) is a critical member of the STAT protein family. It weighs approximately 85 kDa. STAT4 primarily functions as a transcription factor activated by phosphorylation through Janus kinases (JAKs) in response to cytokine signaling. This protein facilitates the translocation into the nucleus where it promotes the transcription of specific genes. STAT4 expression occurs mainly in T cells and natural killer (NK) cells indicating its role in the immune response.

Biological function summary

STAT4 is integral to the differentiation of naïve T cells into T helper 1 (Th1) cells. These Th1 cells play a central role in orchestrating immune responses against intracellular pathogens. STAT4 activation requires two distinct steps involving JAK kinases and subsequent binding to DNA regulatory elements of target genes. STAT4 does not function as part of a large protein complex but rather operates as a dimer following phosphorylation.

Pathways

STAT4 participates strongly in the JAK-STAT signaling pathway fundamental for immune cell maturation and response. The JAK-STAT pathway regulates several biological processes including proliferation and inflammation. STAT4 works closely with STAT1 in promoting Th1 differentiation while also interacting with other proteins like IL-12 to modulate immune activity. These interactions emphasize its influence in immune responses and could affect inflammatory pathways.

STAT4 exhibits associations with autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant STAT4 activity can lead to dysregulated immune responses making it a significant target in these disorders. In the context of rheumatoid arthritis altered STAT4 signaling enhances inflammation through its connection with other immune proteins like IL-23. Understanding STAT4's role in these diseases may offer avenues for therapeutic interventions and better management of immune-related conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response (PubMed : 10961885, PubMed : 37256972, PubMed : 8943379). Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma (PubMed : 12213961, PubMed : 35614130). Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps (By similarity). After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated (PubMed : 10415122, PubMed : 7638186). Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35 (PubMed : 11114383, PubMed : 34508746). Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter (By similarity). In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation (PubMed : 26990433).

See full target information STAT4 phospho Y693

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:81 PubMed38360723

2024

CD24 induced cellular quiescence-like state and chemoresistance in ovarian cancer cells via miR-130a/301a-dependent CDK19 downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonsue Jang,Suki Kang,Hyun Ho Han,Baek Gil Kim,Nam Hoon Cho

EBioMedicine 86:104312 PubMed36335669

2022

Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shihui Xu,Xiaobing Yang,Qingzhou Chen,Zhuoliang Liu,Ying Chen,Xiaotian Yao,An Xiao,Jianwei Tian,Liling Xie,Miaomiao Zhou,Zheng Hu,Fengxin Zhu,Xin Xu,Fanfan Hou,Jing Nie

Journal of translational medicine 19:469 PubMed34798872

2021

Overexpression of E3 ubiquitin ligase Cbl attenuates endothelial dysfunction in diabetes mellitus by inhibiting the JAK2/STAT4 signaling and Runx3-mediated H3K4me3.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Jin,Liangyan Lin,Tiantian Zhao,Xiaoyan Yao,Yaqin Teng,Dongdong Zhang,Yongjun Jin,Meizi Yang

Scientific reports 10:13915 PubMed32807859

2020

Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Xu,Nan Wu,Zhihui Min,Zheng Li,Tao Zhu,Chunfang Liu,Yuzhen Zeng,Juan Song,Ruolin Mao,Hong Ji,Zhilong Jiang,Zhihong Chen

International immunopharmacology 86:106685 PubMed32570032

2020

Baicalin suppresses Th1 and Th17 responses and promotes Treg response to ameliorate sepsis-associated pancreatic injury via the RhoA-ROCK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pingping Liu,Zhenghui Xiao,Haipeng Yan,Xiulan Lu,Xinping Zhang,Lan Luo,Caixia Long,Yimin Zhu

Neuropsychiatric disease and treatment 16:891-900 PubMed32308392

2020

Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejing Yue,Lixia Liu,Haiqing Yan,Yongkun Gui,Jun Zhao,Ping Zhang

Journal of cellular and molecular medicine 23:5588-5599 PubMed31251471

2019

Bryostatin-1 ameliorated experimental colitis in Il-10 Mice by protecting the intestinal barrier and limiting immune dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lugen Zuo,Jing Li,Sitang Ge,Yuanyuan Ge,Mengdi Shen,Yan Wang,Changmin Zhou,Rong Wu,Jianguo Hu

Journal of ethnopharmacology 241:111980 PubMed31146000

2019

A lipid-soluble extract of Pinellia pedatisecta Schott enhances antitumor T cell responses by restoring tumor-associated dendritic cell activation and maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Yumeng Wang,Haixia Huang,Sheng Yao,Guiling Li,Congjian Xu,Yang Ye,Suiqi Gui

Nature communications 9:2745 PubMed30013031

2018

Act1 is a negative regulator in T and B cells via direct inhibition of STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Cun-Jin Zhang,Chenhui Wang,Meiling Jiang,Chunfang Gu,Jianxin Xiao,Xing Chen,Bradley N Martin,Fangqiang Tang,Erin Yamamoto,Yibo Xian,Han Wang,Fengling Li,R Balfour Sartor,Howard Smith,M Elaine Husni,Fu-Dong Shi,Ji Gao,Julie Carman,Ashok Dongre,Susan C McKarns,Ken Coppieters,Trine N Jørgensen,Warren J Leonard,Xiaoxia Li

Innate immunity 22:647-657 PubMed27670945

2016

Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli.

Applications

Species

Mouse

Yishan Chuang,Michelle E Hung,Brianne K Cangelose,Joshua N Leonard

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-STAT4 (phospho Y693) 抗体

Anti-STAT4 (phospho Y693) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT4 (phospho Y693) antibody (AB28815)

Western blot - Anti-STAT4 (phospho Y693) antibody (AB28815)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (12)

代替製品

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise