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Signal Transduction Signaling Pathway Nuclear Signaling STATs
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-STAT2 抗体 [Y141] (ab32367)

  • Datasheet
  • SDS
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Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)
  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
  • Anti-STAT2 antibody [Y141] (ab32367)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y141] to STAT2
  • Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

こちらの製品もご検討ください

一次抗体
Product image
Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] - BSA and Azide free (ab219728)
ノックアウト
Product image
Human STAT2 knockout A549 cell lysate (ab257185)
ノックアウト
Product image
Human STAT2 knockout HeLa cell line (ab261819)

関連製品

製品の概要

  • 製品名

    Anti-STAT2 antibody [Y141]
    STAT2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [Y141] to STAT2
  • 由来種

    Rabbit
  • 特異性

    This antibody detects both long and short forms of STAT2.
  • アプリケーション

    適用あり: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human STAT2 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール

    • WB: A549, K562, HAP1, Ramos, HeLa and THP1 cell lysates; Mouse and Rat brain tissue lysates ICC/IF: THP-1 cells IHC-P: Human hepatocellular carcinoma and thyroid cancer tissue, Mouse and Rat liver Tissue Flow Cyt (intra): Ramos cells
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    Y141
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

関連製品

  • Alternative Versions

    • Anti-STAT2 antibody [Y141] - BSA and Azide free (ab239811)
    • Alexa Fluor® 568 Anti-STAT2 antibody [Y141] (ab312625)
    • Alexa Fluor® 750 Anti-STAT2 antibody [Y141] (ab321593)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STAT2 knockout HeLa cell line (ab261819)
    • Human STAT2 knockout A549 cell line (ab267004)
    • Human STAT2 knockout A549 cell line (ab267005)
    • Human STAT2 knockout A549 cell line (ab267006)
  • KO cell lysates

    • Human STAT2 knockout HeLa cell lysate (ab257182)
    • Human STAT2 knockout A549 cell lysate (ab257183)
    • Human STAT2 knockout A549 cell lysate (ab257184)
    • Human STAT2 knockout A549 cell lysate (ab257185)
  • Recombinant Protein

    • Recombinant human STAT2 protein (ab81880)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32367の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Flow Cyt (Intra)
1/60.
ICC/IF
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

特記事項
WB
1/5000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Flow Cyt (Intra)
1/60.
ICC/IF
1/50.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

ターゲット情報

  • 機能

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • 配列類似性

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻訳後修飾

    Tyrosine phosphorylated in response to IFN-alpha.
  • 細胞内局在

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Target information above from: UniProt accession P52630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6773 Human
    • Entrez Gene: 20847 Mouse
    • Entrez Gene: 288774 Rat
    • Omim: 600556 Human
    • SwissProt: P52630 Human
    • SwissProt: Q9WVL2 Mouse
    • Unigene: 530595 Human
    • Unigene: 293120 Mouse
    • Unigene: 471333 Mouse
    see all
  • 別名

    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

画像

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab32367 was shown to react with STAT2 in A549 wild-type cells in western blot with loss of signal observed in STAT2 knockout cell line ab267004 (STAT2 knockout cell lysate ab257183). Wild-type and STAT2 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk before incubation with ab32367 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32367 was shown to react with STAT2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab267005 (knockout cell lysate ab257184) was used. Wild-type A549 and STAT2 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAT2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32367 was shown to react with STAT2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261819 (knockout cell lysate ab257182) was used. Wild-type HeLa and STAT2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/5000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : STAT2 knockout A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 98 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab32367 Anti-STAT2 antibody [Y141] was shown to specifically react with STAT2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267006 (knockout cell lysate ab257185) was used. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STAT2 knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : THP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 98 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32367 (unpurified) observed at 97 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32367 was shown to recognize STAT2 in wild-type HAP1 cells as signal was lost at the expected MW in STAT2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. Ab32367 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)
    Flow Cytometry (Intracellular) - Anti-STAT2 antibody [Y141] (ab32367)

    Intracellular Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling STAT2 with purified ab32367 at 1/60 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody [Y141] (ab32367)

    Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling STAT2 with purified ab32367 at 1/50 dilution (10 µg/mL). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    Western blot - Anti-STAT2 antibody [Y141] (ab32367)
    All lanes : Anti-STAT2 antibody [Y141] (ab32367) at 1/1000 dilution (Purified)

    Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 98 kDa
    Observed band size: 113,97 kDa why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling STAT2 with purified ab32367 at 1/100 dilution (6.22 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody [Y141] (ab32367)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ab32367 (unpurified). Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-STAT2 antibody [Y141] (ab32367)
    Anti-STAT2 antibody [Y141] (ab32367)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (10)

ab32367 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32367 は 10 報の論文で使用されています。

  • Zhao Y  et al. EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8. J Virol 97:e0102823 (2023). PubMed: 37772822
  • Ji W  et al. TP53TG1/STAT axis is involved in the development of colon cancer through affecting PD-L1 expression and immune escape mechanism of tumor cells. Am J Cancer Res 13:5218-5235 (2023). PubMed: 38058799
  • Pan J  et al. Identification of cancer-associated fibroblasts subtypes in prostate cancer. Front Immunol 14:1133160 (2023). PubMed: 37033924
  • Lv C  et al. Narciclasine targets STAT3 via distinct mechanisms in tamoxifen-resistant breast cancer cells. Mol Ther Oncolytics 24:340-354 (2022). PubMed: 35118192
  • Xu S  et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
  • Lu Y  et al. Manipulation of innate immune signaling pathways by SARS-CoV-2 non-structural proteins. Front Microbiol 13:1027015 (2022). PubMed: 36478862
  • Jin J  et al. Small-molecule PROTAC mediates targeted protein degradation to treat STAT3-dependent epithelial cancer. JCI Insight 7:N/A (2022). PubMed: 36509291
  • Wang R  et al. ORF3a Protein of Severe Acute Respiratory Syndrome Coronavirus 2 Inhibits Interferon-Activated Janus Kinase/Signal Transducer and Activator of Transcription Signaling via Elevating Suppressor of Cytokine Signaling 1. Front Microbiol 12:752597 (2021). PubMed: 34650546
  • Qu T  et al. ISG15 induces ESRP1 to inhibit lung adenocarcinoma progression. Cell Death Dis 11:511 (2020). PubMed: 32641707
  • Yin D  et al. Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124. Cell Physiol Biochem 50:2188-2202 (2018). PubMed: 30415256

レビューと Q&A

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Western blot abreview for Anti-STAT2 antibody [Y141]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
25 µg
Specification
hek293
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Robert Kupp

Verified customer

投稿 Oct 20 2020

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