Anti-STAT2 抗体 (ab245394)
Key features and details
- Rabbit polyclonal to STAT2
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-STAT2 antibody
STAT2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STAT2 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human STAT2 aa 725-775. The exact sequence is proprietary. NP_005410.1
Database link: P52630 -
ポジティブ･コントロール
- WB: Jurkat, HEK-293T and HeLa whole cell lysates. IP: Jurkat whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245394 was affinity purified using an epitope specific to STAT2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245394の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 98 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 98 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated in response to IFN-alpha. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta. - Information by UniProt
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参照データベース
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
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別名
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT2 antibody (ab245394)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for STAT2 using ab245394 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
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Western blot - Anti-STAT2 antibody (ab245394)All lanes : Anti-STAT2 antibody (ab245394) at 0.04 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 98 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-STAT2 antibody (ab245394)STAT2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245394 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245394 at 1 µg/ml.
Lane 1: ab245394 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245394 は論文での使用が確認できていません。