Anti-STAT1 (phospho S727) 抗体 [EPR3146]
Anti-STAT1 (phospho S727) antibody [EPR3146]
- 20ul selling size
- Recombinant
- Advanced Validation
- RabMAb
- 詳細を見る
4
(1 Review)
|
(47 Publications)
Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) is a rabbit monoclonal antibody detecting STAT1 in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
別名を表示する
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1
- WB
Lab
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Blocking buffer : 2% BSA/TBST
Dilution buffer : 2% BSA/TBST
All lanes:
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/5000 dilution
Lane 1:
Untreated HeLa whole cell lysate at 10 µg
Lane 2:
HeLa whole cell lysate treated with etoposide at 10 µg
Lane 3:
HeLa whole cell lysate treated with etoposide, followed by membrane treatment with phosphatase at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 90 kDa
false
- WB
Lab
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
The lower section shows STAT1 detected with ab92506, anti-STAT1 antibody, to confirm that the same amount of lysate is used in each lane.
Blocking and dilution biffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/10000 dilution
Lane 1:
Untreated HeLa (human cervix adenocarcinoma) at 10 µg
Lane 2:
HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution
Predicted band size: 87 kDa
Observed band size: 91 kDa
true
Exposure time: 30s
- WB
Supplier Data
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as a GAPDH loading at 1000000 dilution.
All lanes:
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution
Lane 1:
Untreated HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
HEK-293 treated with 50 ng/ml IFN beta for 3 hours whole cell lysate at 20 µg
Lane 3:
Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4:
RAW 264.7 treated with 100 ng/ml IFN alpha for 24 hours whole cell lysate at 20 µg
Lane 5:
Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 6:
NIH/3T3 treated with 100 ng/ml IFN gamma for 48 hours whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 91 kDa,37 kDa
Observed band size: 91 kDa,37 kDa
false
Exposure time: 3s
- ChIC/CUT&RUN-seq
Supplier Data
ChIC/CUT&RUN sequencing - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) treated with IFN gamma (50ng/ml 1h) cells and 5µg of ab109461 [EPR3146]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Unpurified ab109461, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Immunohistochemical staining of paraffin embedded mouse colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Immunohistochemical staining of paraffin embedded rat colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
- WB
Lab
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Blocking buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg
Lane 3:
Mouse brain lysate at 15 µg
Lane 4:
Mouse brain lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 91 kDa
false
- WB
Lab
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Blocking buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/1000 dilution
Lane 1:
Rat brain lysate at 15 µg
Lane 2:
Rat brain lysate (treated with Alkaline Phosphatase for 1 hour) at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 91 kDa
false
- Dot
Lab
Dot Blot - Anti-STAT1 (phospho S727) antibody [EPR3146] (AB109461)
Dot Blot analysis of Lane 1 : STAT1 (pS727) phospho peptide and Lane 2 : STAT1 non-phospho peptide, labeling STAT1 (phospho S727) with ab109461 at 1/1000 dilution. 5% NFDM/TBST was used as the blocking and diluting buffer. ab97051, a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at 1/100000 dilution. Exposure time : 3 minutes.
関連する標識済み抗体及び組成の異なる製品 (1)
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Anti-STAT1 (phospho S727) antibody [EPR3146] - BSA and Azide free
Reactivity data
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製品の詳細
What is this antibody validated in?
Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of STAT1?
Anti-STAT1 (phospho S727) [EPR3146] (ab109461) specifically detects a band for STAT1 (UniProt: P42224) at a molecular weight of 87kDa.
Trusted by the scientific community
Anti-STAT1 (phospho S727) [EPR3146] (ab109461) was first used in a scientific publication in 2011 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR3146] also available for your convenience: ab109461, Carrier free - ab215820
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存期間
短期保存温度
長期保存温度
分注に関する情報
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.
Pathways
The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.
製品プロトコール
- Visit the General protocols
- Visit the Troubleshooting
- Download chicCutRunSequencingBooklet|en
ターゲットの情報
文献 (47)
Recent publications for all applications. Explore the full list and refine your search
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