AB3987

Anti-STAT1 抗体 [SM1]

Name: Anti-STAT1 抗体 [SM1] (ab3987) | Abcam
Brand: Abcam Limited
SKU: AB3987
Price: 76000 JPY
Availability: InStock
Rating: 3 (3 reviews)

Anti-STAT1 antibody [SM1]

(3 Reviews)

(31 Publications)

Mouse Monoclonal STAT1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Human STAT1 aa 700 to C-terminus.

別名を表示する

Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

3 Images

Flow Cytometry - Anti-STAT1 antibody [SM1] (AB3987)

Flow Cyt

Unknown

Flow Cytometry - Anti-STAT1 antibody [SM1] (AB3987)

Overlay histogram showing HeLa cells stained with ab3987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3987, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Supplier Data

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Western blotting analysis of STAT1 using ab3987 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions.

Nitrocellulose membrane was probed with 2 μg/ml of ab3987 followed by IRDye800-conjugated anti-mouse secondary antibody.

All lanes:

Western blot - Anti-STAT1 antibody [SM1] (ab3987) at 2 µg/mL

Lane 1:

HeLa cell lysate (reducing conditions)

Lane 2:

MCF-7 cell lysate (reducing conditions)

Lane 3:

Cos-7 cell lysate (reducing conditions)

Secondary

All lanes:

IRDye800-conjugated anti-mouse

Predicted band size: 87 kDa

Observed band size: 90 kDa

false

Unknown

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727) by the antibody PSM1 (upper panel) and total STAT 1 level by the antibody SM1 (lower panel).

All lanes:

Western blot - Anti-STAT1 antibody [SM1] (ab3987)

Predicted band size: 87 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

SM1

アイソタイプ

IgG2b

キャリアフリー

交差種

Human

アプリケーション

WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human STAT1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P42224

特異性

The antibody recognizes an epitope included within amino acids 721-733 of the 91 kDa STAT 1 protein.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

精製に関する特記事項

Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.

Biological function summary

The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.

Pathways

The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.

STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors (PubMed : 12764129, PubMed : 12855578, PubMed : 15322115, PubMed : 23940278, PubMed : 34508746, PubMed : 35568036, PubMed : 9724754). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed : 28753426, PubMed : 35568036). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state (PubMed : 28753426, PubMed : 35568036). In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed : 26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state (PubMed : 8156998). Becomes activated in response to KITLG/SCF and KIT signaling (PubMed : 15526160). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 19088846). Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylated at Thr-749 by IKBKB which promotes binding of STAT1 to the 5'-TTTGAGGC-3' sequence in the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 (PubMed : 32209697). Phosphorylation at Thr-749 also promotes binding of STAT1 to the 5'-TTTGAGTC-3' sequence in the IL12B promoter and activation of IL12B transcription (PubMed : 32209697). Involved in food tolerance in small intestine : associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine (By similarity).

See full target information STAT1

style

left-column

文献 (31)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:82 PubMed38512482

2024

FAM83D acts as an oncogene by regulating cell cycle progression via multiple pathways in synovial sarcoma: a potential novel downstream target oncogene of anlotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Mei Liu,Ying Yuan,Lei Jin

Frontiers in immunology 15:1331731 PubMed38384473

2024

ISG15/USP18/STAT2 is a molecular hub regulating IFN I-mediated control of Dengue and Zika virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Constanza Eleonora Espada,Edroaldo Lummertz da Rocha,Taissa Ricciardi-Jorge,Adara Aurea Dos Santos,Zamira Guerra Soares,Greicy Malaquias,Daniel Oliveira Patrício,Edgar Gonzalez Kozlova,Paula Fernandes Dos Santos,Juliano Bordignon,Thomas J Sanford,Teodoro Fajardo,Trevor R Sweeney,André Báfica,Daniel Santos Mansur

BMC cancer 23:1114 PubMed37968576

2023

ESCO2 promotes hypopharyngeal carcinoma progression in a STAT1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Hu,Jing Yan,Yijie Chen,Xiaohui Li,Liu Yang,Haiyu Di,Huihui Zhang,Yewen Shi,Junjie Zhao,Yanxia Shi,Yinglong Xu,Xiaoyong Ren,Zhenghui Wang

American journal of cancer research 13:5218-5235 PubMed38058799

2023

TP53TG1/STAT axis is involved in the development of colon cancer through affecting PD-L1 expression and immune escape mechanism of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenmin Ji,Wenyan Wang,Yanfang Wei

mBio 14:e0093423 PubMed37732809

2023

PKR-mediated stress response enhances dengue and Zika virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Taissa Ricciardi-Jorge,Edroaldo Lummertz da Rocha,Edgar Gonzalez-Kozlova,Gabriela Flavia Rodrigues-Luiz,Brian J Ferguson,Trevor Sweeney,Nerea Irigoyen,Daniel Santos Mansur

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 24:927-940 PubMed35107757

2022

STAT1/IFIT2 signaling pathway is involved in PD-L1-mediated epithelial-to-mesenchymal transition in human esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J Chen,Y Liu,Y Zhu,Y Chen,J Feng,T Jiang,X Zheng,L Chen,J Jiang

Open medicine (Warsaw, Poland) 16:1642-1652 PubMed34761115

2021

miR-150-5p affects AS plaque with ASMC proliferation and migration by STAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Bian,Wenqiang Cai,Hongying Lu,Shuhong Tang,Keqin Yang,Yan Tan

Life sciences 277:119419 PubMed33785336

2021

LncRNA DLEU2 is activated by STAT1 and induces gastric cancer development via targeting miR-23b-3p/NOTCH2 axis and Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchun Li,Zhen Zhang,Zhaosheng Chen,Bin Liu,Honglei Wu

American journal of translational research 13:210-222 PubMed33527019

2021

Schisandrin C attenuates renal damage in diabetic nephropathy by regulating macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jingqiu Cui,Ming Liu,Yingqi Shao,Xiaoying Dong

Cell death & disease 11:693 PubMed32826878

2020

TLR4 promotes microglial pyroptosis via lncRNA-F630028O10Rik by activating PI3K/AKT pathway after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Xu,Jin Wang,Jianyuan Jiang,Jian Song,Wei Zhu,Fan Zhang,Minghao Shao,Haocheng Xu,Xiaosheng Ma,Feizhou Lyu

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-STAT1 抗体 [SM1]

Anti-STAT1 antibody [SM1]

Flow Cytometry - Anti-STAT1 antibody [SM1] (AB3987)

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Western blot - Anti-STAT1 antibody [SM1] (AB3987)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (31)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise