Name: Anti-StAR antibody [EP7639]
Brand: RabMAb
SKU: ab133657
Rating: 4 (2 reviews)

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Western blot - Anti-StAR antibody [EP7639] (ab133657)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP7639] to StAR
Suitable for: WB, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-StAR antibody [EP7639]
StAR 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP7639] to StAR
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: IP
種交差性

交差種: Human
免疫原

Synthetic peptide within Human StAR aa 250 to the C-terminus. The exact sequence is proprietary.
ポジティブ･コントロール
- Human adrenal gland lysate; Human adrenal gland tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Tissue culture supernatant
ポリ/モノ

モノクローナル
クローン名

EP7639
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-StAR antibody [EP7639] - BSA and Azide free (ab244236)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human StAR protein (ab115707)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab133657の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(2)	1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

追加情報

Is unsuitable for IP.

機能

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
組織特異性

Expressed in gonads, adrenal cortex and kidney.
パスウェイ

Steroid metabolism; cholesterol metabolism.
関連疾患

Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
配列類似性

Contains 1 START domain.
細胞内局在

Mitochondrion.
Target information above from: UniProt accession P49675 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6770 Human
- Omim: 600617 Human
- SwissProt: P49675 Human
- Unigene: 521535 Human
別名
- Cholesterol trafficker antibody
- Luteinizing hormone induced protein antibody
- mitochondrial antibody
- Mitochondrial steroid acute regulatory protein antibody
- StAR antibody
- StAR related lipid transfer (START) domain containing 1 antibody
- STAR_HUMAN antibody
- StARD1 antibody
- START domain containing 1 antibody
- START domain containing protein 1 antibody
- START domain-containing protein 1 antibody
- Steroid acute regulatory protein antibody
- Steroidogenic acute regulator antibody
- Steroidogenic acute regulatory protein antibody
- Steroidogenic acute regulatory protein mitochondrial antibody
see all

Western blot - Anti-StAR antibody [EP7639] (ab133657)

Anti-StAR antibody [EP7639] (ab133657) at 1/10000 dilution + Human adrenal gland lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labelling StAR with ab133657 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Normal testis tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Adrenocortical carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody [EP7639] (ab133657)

ab133657 showing positive staining in Sertoli-Leydig cell tumor tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-StAR antibody [EP7639] (ab133657)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab133657 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab133657 は 11 報の論文で使用されています。

Källsten L et al. Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies. Cells 11:N/A (2022). PubMed: 36230992
Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
Ma J et al. Single-cell transcriptomic analysis of endometriosis provides insights into fibroblast fates and immune cell heterogeneity. Cell Biosci 11:125 (2021). PubMed: 34233737
Bose HS et al. Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB). Mol Cell Biol 41:e0035721 (2021). PubMed: 34460330
Asiabi P et al. Immunodetection and quantification of enzymatic markers in theca cells: the early process of ovarian steroidogenesis†. Biol Reprod 102:145-155 (2020). PubMed: 31504196

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)

Gel Running Conditions

Reduced Denaturing (15)

Loading amount

25 µg

Specification

Human adrenal corticocarcinoma H295R cells

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

投稿 Jun 27 2017

Application

Western blot

Sample

Sheep Tissue lysate - whole (ovine theca cells, ov-placenta tissue, ov-liver ti)

Gel Running Conditions

Reduced Denaturing (10% PAG)

Loading amount

20 µg

Specification

ovine theca cells, ov-placenta tissue, ov-liver ti

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Barbara Makela

Verified customer

投稿 Jun 23 2017

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

パスウェイ

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (11)

レビューと Q&A

Western blot abreview for Anti-StAR antibody [EP7639]

Western blot abreview for Anti-StAR antibody [EP7639]