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Anti-StAR 抗体 (ab96637)

Reviews (3)Q&A (2)References (24)
Western blot - Anti-StAR antibody (ab96637)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody (ab96637)
  • Immunocytochemistry - Anti-StAR antibody (ab96637)
  • Western blot - Anti-StAR antibody (ab96637)
  • Western blot - Anti-StAR antibody (ab96637)

Key features and details

  • Rabbit polyclonal to StAR
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-StAR antibody [EP7639] (ab133657)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-StAR antibody
    StAR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to StAR

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Cow, Dog, Pig

  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab96637の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
WB (3)
1/100 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P
1/100 - 1/1000.
特記事項
ICC
Use at an assay dependent concentration.
WB
1/100 - 1/10000. Predicted molecular weight: 32 kDa.
IHC-P
1/100 - 1/1000.

ターゲット情報

  • 機能

    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

  • 組織特異性

    Expressed in gonads, adrenal cortex and kidney.

  • パスウェイ

    Steroid metabolism; cholesterol metabolism.

  • 関連疾患

    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.

  • 配列類似性

    Contains 1 START domain.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cholesterol trafficker antibody
      • Luteinizing hormone induced protein antibody
      • mitochondrial antibody
      • Mitochondrial steroid acute regulatory protein antibody
      • StAR antibody
      • StAR related lipid transfer (START) domain containing 1 antibody
      • STAR_HUMAN antibody
      • StARD1 antibody
      • START domain containing 1 antibody
      • START domain containing protein 1 antibody
      • START domain-containing protein 1 antibody
      • Steroid acute regulatory protein antibody
      • Steroidogenic acute regulator antibody
      • Steroidogenic acute regulatory protein antibody
      • Steroidogenic acute regulatory protein mitochondrial antibody
      see all

    画像

    • Western blot - Anti-StAR antibody (ab96637)
      Western blot - Anti-StAR antibody (ab96637)
      Anti-StAR antibody (ab96637) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg

      Secondary
      HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 32 kDa



      12% SDS-PAGE

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody (ab96637)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-StAR antibody (ab96637)

      Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining StAR protein at cytosol with ab96637 at 1/500.

      Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

    • Immunocytochemistry - Anti-StAR antibody (ab96637)
      Immunocytochemistry - Anti-StAR antibody (ab96637)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate labeling StAR protein at mitochondria with ab96637 at 1/500 dilution. Blue: Hoechst 33342 staining.

    • Western blot - Anti-StAR antibody (ab96637)
      Western blot - Anti-StAR antibody (ab96637)
      All lanes : Anti-StAR antibody (ab96637) at 1/5000 dilution

      Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : Transfected HEK-293T whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG antibody

      Predicted band size: 32 kDa



      12% SDS-PAGE

    • Western blot - Anti-StAR antibody (ab96637)
      Western blot - Anti-StAR antibody (ab96637)Image courtesy of an anonymous Abreview.
      All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution

      Lane 1 : Whole tissue lysate prepared from murine adrenal gland
      Lane 2 : Whole tissue lysate prepared from rat adrenal gland

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution

      Developed using the ECL technique.

      Predicted band size: 32 kDa


      Exposure time: 20 seconds

      See Abreview

    参考文献 (24)

    ab96637 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab96637 は 24 報の論文で使用されています。

    • Manna PR  et al. Expression and Function of StAR in Cancerous and Non-Cancerous Human and Mouse Breast Tissues: New Insights into Diagnosis and Treatment of Hormone-Sensitive Breast Cancer. Int J Mol Sci 24:N/A (2023). PubMed: 36614200
    • Wang Y  et al. Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum. Sci Rep 13:2940 (2023). PubMed: 36808140
    • Plewes MR  et al. Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum. Life Sci Alliance 6:N/A (2023). PubMed: 37188480
    • Minowa K  et al. Insulin dysregulation drives mitochondrial cholesterol metabolite accumulation: initiating hepatic toxicity in nonalcoholic fatty liver disease. J Lipid Res 64:100363 (2023). PubMed: 36966904
    • Mohr MA  et al. Puberty enables oestradiol-induced progesterone synthesis in female mouse hypothalamic astrocytes. J Neuroendocrinol 34:e13082 (2022). PubMed: 35000221
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-StAR antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Adrenal)
    Loading amount
    50 µg
    Specification
    Adrenal
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 23 2011

    Western blot abreview for Anti-StAR antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    50 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 20 2010

    Western blot abreview for Anti-StAR antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1)
    Loading amount
    25 µg
    Specification
    THP-1
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 29 2010

    Question

    Interested in using ab96637 with pig samples.

    Read More
    Answer

    Thank you very much for your interest in ab96637. Sinceab96637 has not been tested in pig, I can offer a discount off a future purchase if you buy ab96637 now, test it in pig, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab96637 in pig. I will then send a discount code. This code must be issued before purchasing ab96637 so please wait for my reply before ordering. 2. Purchase ab96637 either by phone, fax, or online (www.abcam.com). 3. Test it in pig. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96637 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    DESCRIPTION OF THE PROBLEM Non-specific staining Staining pattern is absent in the paraffin embedded sections of mice testis SAMPLE Fresh testis Bouin’s / Davidson’s fixed paraffin embedded sections. PRIMARY ANTIBODY Concentration or dilution 1:25. 1:50 and 1:100 Diluent buffer 1% BSA in PBS Incubation time overnight Incubation temperature 4 ° C DETECTION METHOD hrp POSITIVE AND NEGATIVE CONTROLS USED Positive control- Anti- P450 SCC antibody, Rabbit polyclonal (1:100) ANTIBODY STORAGE CONDITIONS 4 degC FIXATION OF SAMPLE Fixation: Yes/No Not necessary Fixative agent and concentration Fixation time Fixation temperature ANTIGEN RETRIEVAL no PERMEABILIZATION STEP Yes/No Not necessary Permeabilization agent and concentration Permeabilization time Permeabilization temperature BLOCKING CONDITIONS Yes/No Blocking agent and concentration 15% FBS Blocking time 30 minutes Blocking temperature RT Endogenous peroxidases blocked? Yes/No yes Endogenous biotins blocked? Yes/No no SECONDARY ANTIBODY Concentration or dilution 1:25. 1:50 and 1:100 Diluent buffer 1% BSA in PBS Incubation time overnight Incubation temperature 4 ° C Species Goat Anti-rabbit IgG Reacts against rabbit Concentration or dilution 1:100 Diluent buffer in PBS Incubation time I hour Incubation temperature: 37° celsius Fluorochrome or enzyme conjugate Enzyme conjugate HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Concentration of primary antibody ADDITIONAL NOTES After primary antibody After secondary antibody Buffer Buffer pbs Buffer pbs Number of washes 3 Number of washes Buffer 3

    Read More
    Answer

    Thank you for submitting your completed questionnaire. To our knowledge ab96637 has not been previously tested in IHC-P. Thus is may be that this antibody does not function effectively in this application. However, results may be improved by doing an antigen retrieval step, for example a heat induced epitope retreival with pH 9.0 buffer. An example of this protocol, along with a recipe for this retrieval buffer can be found in our IHC protocol: https://www.abcam.com/index.html?pageconfig=resource&rid=11384 I hope this is helpful. I look forward to hearing if this tip proves useful. Please contact me again if you have any further questions.

    Read More

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