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AB20920

Anti-Staphylococcus aureus 抗体

Anti-Staphylococcus aureus antibody

4

(8 Reviews)

|

(47 Publications)

Anti-Staphylococcus aureus antibody (ab20920) is a rabbit polyclonal antibody detecting Staphylococcus aureus in IHC-Fr, ICC/IF. Suitable for Staphylococcus aureus.

- Over 40 publications
- Trusted since 2005

別名を表示する

IgG binding protein A, Immunoglobulin G binding protein A

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)
  • ICC/IF

AbReview27826****

Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)

ab20920 staining Staphylococcus aureus in human keratinocytes by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in methanol, permeabilized using 0.1% saponin/ PBS, blocked with 4% BSA for 30 minutes at 25°C and then incubated with ab20920 at 10μg/ml for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

Red : cadherin
Blue : DAPI
Green : anti-Staphylococcus aureus (ab20920)

Image courtesy of an anonymous Abreview.

Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (AB20920)
  • IHC-Fr

AbReview9540****

Immunohistochemistry (Frozen sections) - Anti-Staphylococcus aureus antibody (AB20920)

ab20920 staining an abscess in rat calf tissue prepared as a 20 μm cryoslice. Tissue was fixed in paraformaldehyde, permeabilised with 0,5% Trition X100 in PBS, and blocked with 10% serum plus 1% BSA for 1 hour at room temperature. The primary antibody was diluted 1/500 with PBS plus 3-5% Serum, 0,5% Trition X100 and incubated for 24 hours at 20°C. An Alexa Fluor® 568 goat anti-rabbit was used as the secondary

This image is courtesy of an Abreview submitted by Dr Dagmar Salber

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Staphylococcus aureus

アプリケーション

IHC-Fr, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Soluble and structural antigens of the whole bacterium. Antiserum is not absorbed for related microorganisms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Use with acetone fixed tissues.</p>" } } }
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製品の詳細

What is this antibody validated in?
Anti-Staphylococcus aureus antibody (ab20920) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Staphylococcus aureus samples.

Trusted by the scientific community
Anti-Staphylococcus aureus (ab20920) was first used in a scientific publication in 2005 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Staphylococcus aureus (ab20920) has over 5 independent reviews from customers.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.1% Sodium azide Constituents: 0.0268% PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Staphylococcus aureus also known as "aureus" or "staph" is a gram-positive bacterium with a molecular mass ranging around 2.8 million daltons. It is found mainly in the nasal passages and on the skin of humans. This bacterium has a thick peptidoglycan layer making it resistant to certain environmental pressures. The structure of its proteins like Protein A plays an important role in immune evasion. Staphylococcus aureus also expresses a variety of toxins and enzymes that aid in its survival and pathogenicity.
Biological function summary

Staphylococcus aureus acts as an opportunistic pathogen that can cause infections when the host's immune system is weakened. It is not part of a specific protein complex but utilizes a range of virulence factors to establish infections. The bacterium produces biofilms which provide a protective environment against host defenses and antimicrobial agents. Its ability to quickly adapt to different environments makes it a major cause of both nosocomial and community-acquired infections.

Pathways

The metabolic activities of Staphylococcus aureus are involved in two significant pathways: the peptidoglycan biosynthesis and the toxin production pathway. Peptidoglycan biosynthesis is essential for cell wall integrity while toxin production pathways lead to the synthesis of critical virulence factors like alpha-hemolysin. Key proteins involved in these pathways include PBP2a which is significant for methicillin resistance and hemolysins that damage host tissues.

Staphylococcus aureus is connected to skin infections and pneumonia. The bacterium is notorious for causing conditions such as MRSA (methicillin-resistant Staphylococcus aureus) infections which are challenging to treat due to antibiotic resistance. Protein A interaction with the Fc region of immunoglobulin contributes to the evasion of immune responses and chronic infection persistence. These properties make staph infections a significant concern in both hospital and community settings.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information Staphylococcus aureus
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文献 (47)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 15: PubMed38004123

2023

Nanoparticles of K8 Reduce Respiratory Infection and Tumor Necrosis Factor Alpha- and Interferon Gamma-Induced Lung Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jonghyo Hong,Minseong Son,Jaeeun Sin,Hangeun Kim,Dae-Kyun Chung

Frontiers in cellular and infection microbiology 13:1182480 PubMed37293208

2023

A comparative F-FDG and an anti-PD-L1 probe PET/CT imaging of implant-associated osteomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Qi Ren,Yuan Ma,Li-Lan Fu,Kong-Zhen Hu,Hao-Ran Liang,Bin Yu,Gang-Hua Tang

JACC. Basic to translational science 8:1439-1453 PubMed38093743

2023

Protective Effect of Ticagrelor Against Infective Endocarditis Induced by Virulent in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Oury,Severien Meyers,Nicolas Jacques,Kirsten Leeten,Zheshen Jiang,Lucia Musumeci,Marleen Lox,Margaux Debuisson,Eric Goffin,Bernard Pirotte,Philippe Delvenne,Alain Nchimi,Cédric Hubert,Mélanie Heptia,Philippe Hubert,Marijke J E Kuijpers,Thomas Vanassche,Kimberly Martinod,Peter Verhamme,Patrizio Lancellotti

Scientific reports 12:21846 PubMed36528648

2022

Development of an implantable three-dimensional model of a functional pathogenic multispecies biofilm to study infected wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Camila Cárdenas-Calderón,Valentina Veloso-Giménez,Tamara González,Aniela Wozniak,Patricia García,Sebastián San Martín,Juan F Varas,Ivo Carrasco-Wong,Mario Vera,José Tomás Egaña

BMC biology 20:270 PubMed36464676

2022

Visualizing extracellular vesicle biogenesis in gram-positive bacteria using super-resolution microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Dokyung Jeong,Min Jeong Kim,Yejin Park,Jinkyoung Chung,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

Gut microbes 14:2143220 PubMed36369946

2022

Kidney microbiota dysbiosis contributes to the development of hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Liu,Jing Li,Yamei Zhang,Luyun Fan,Yanli Xia,Yongyang Wu,Junru Chen,Xinyu Zhao,Qiannan Gao,Bing Xu,Chunlai Nie,Zhengyu Li,Aiping Tong,Wenjie Wang,Jun Cai

Frontiers in physiology 13:808272 PubMed35957979

2022

Vitamin D Receptor Genetic Polymorphisms Associate With a Decreased Susceptibility to Extremity Osteomyelitis Partly by Inhibiting Macrophage Apoptosis Through Inhibition of Excessive ROS Production VDR-Bmi1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Qi Zhao,Hao-Yang Wan,Si-Ying He,Han-Jun Qin,Bin Yu,Nan Jiang

Journal of dairy science 105:7705-7718 PubMed35879165

2022

Investigation of the pathophysiology of bacterial mastitis using precision-cut bovine udder slices.

Applications

Unspecified application

Species

Unspecified reactive species

V Filor,B Seeger,N de Buhr,M von Köckritz-Blickwede,M Kietzmann,H Oltmanns,J Meißner

eLife 11: PubMed35723663

2022

NAD kinase promotes pathogenesis by supporting production of virulence factors and protective enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Clarisse Leseigneur,Laurent Boucontet,Magalie Duchateau,Javier Pizarro-Cerda,Mariette Matondo,Emma Colucci-Guyon,Olivier Dussurget

Metabolomics : Official journal of the Metabolomic Society 18:39 PubMed35687250

2022

Bioactive lipid screening during respiratory tract infections with bacterial and viral pathogens in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schultz,Fabian Cuypers,Sebastian B Skorka,Jan Rockstroh,Manuela Gesell Salazar,Jakob Krieger,Dirk Albrecht,Uwe Völker,Sven Hammerschmidt,Michael Lalk,Nikolai Siemens,Karen Methling
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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