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AB151545

Anti-STAM2 抗体 [EPR8688]

Anti-STAM2 antibody [EPR8688]

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(1 Publication)

Rabbit Recombinant Monoclonal STAM2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

別名を表示する

HBP, STAM2, Signal transducing adapter molecule 2, STAM-2, Hrs-binding protein

4 Images
Western blot - Anti-STAM2 antibody [EPR8688] (AB151545)
  • WB

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Western blot - Anti-STAM2 antibody [EPR8688] (AB151545)

All lanes:

Western blot - Anti-STAM2 antibody [EPR8688] (ab151545) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 58 kDa

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Flow Cytometry (Intracellular) - Anti-STAM2 antibody [EPR8688] (AB151545)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-STAM2 antibody [EPR8688] (AB151545)

Overlay histogram showing HeLa cells stained with ab151545 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151545 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody [EPR8688] (AB151545)
  • IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM2 antibody [EPR8688] (AB151545)

Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling STAM2 with ab151545 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-STAM2 antibody [EPR8688] (AB151545)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAM2 antibody [EPR8688] (AB151545)

Immunofluorescent analysis of HepG2 cells labeling STAM2 with ab151545 at 1/100 dilution.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-STAM2 antibody [EPR8688] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR8688

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000 - 1/10000", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

STAM2 also known as STAM2b acts as a signal transducing adaptor molecule. STAM2 has a mass of approximately 69 kDa and is located in the cytoplasm. It expresses widely with notable presence in the brain and immune cells. Mechanically STAM2 contains multiple functional domains including a VHS domain and a UIM domain which participate in binding ubiquitinated proteins. These features allow STAM2 to mediate interactions in cellular processes involving intracellular signaling.
Biological function summary

STAM2 plays a role in the sorting and trafficking of proteins within the cell. It forms part of the ESCRT-0 complex which is important for sorting ubiquitinated cargo into the multivesicular body (MVB) pathway. This pathway is important for the degradation of membrane proteins in lysosomes and regulating various signaling pathways including endocytosis. The involvement of STAM2 in these processes makes it essential for maintaining cellular homeostasis and regulating signal transduction.

Pathways

STAM2 is involved in both the endocytic and lysosomal degradation pathways. Within these pathways STAM2 interacts with Hrs (hepatocyte growth factor-regulated tyrosine kinase substrate) another component of the ESCRT-0 complex to regulate the sorting of endocytic cargo. This interaction is part of a larger network connecting the endocytic pathway with other cellular processes such as ubiquitin-dependent protein degradation. STAM2's functions align it with pathways necessary for proper protein turnover and cellular signaling adjustments.

STAM2's dysregulation has a noted connection to neurodegenerative diseases such as Alzheimer's disease. Changes in protein trafficking and degradation which involve STAM2 function correlate with the buildup of abnormal protein aggregates in neurons. Additionally STAM2 has been implicated in certain cancers where its interaction with proteins such as EGFR (epidermal growth factor receptor) may influence tumor growth and metastasis. Understanding how STAM2 contributes to these conditions could offer insight into potential therapeutic targets.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).
See full target information STAM2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 53:697-706 PubMed33778841

2021

STAM2 knockdown inhibits proliferation, migration, and invasion by affecting the JAK2/STAT3 signaling pathway in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Qi Zhang,Jiakui Liang,Meiyuan Yang,Zheng Wang,Dong Tang,Daorong Wang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

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