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AB244470

Anti-STAM1 抗体

Anti-STAM1 antibody

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(3 Publications)

Rabbit Polyclonal STAM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STAM aa 100-200.

別名を表示する

STAM1, STAM, Signal transducing adapter molecule 1, STAM-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-STAM1 antibody (AB244470)

PFA-fixed, Triton X-100 permeabilized Caco-2 (human colorectal adenocarcinoma cell line) cells stained for STAM1 (green) using ab244470 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB244470)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB244470)

Paraffin-embedded human bone marrow tissue stained for STAM1 using ab244470 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-STAM1 antibody (AB244470)
  • WB

Unknown

Western blot - Anti-STAM1 antibody (AB244470)

All lanes:

Western blot - Anti-STAM1 antibody (ab244470) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Predicted band size: 59 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human STAM aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q92783

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代替製品

Primary Antibodies

AB155527

Anti-STAM1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAM1 antibody (AB155527)

  • 0
  • 0
Primary Antibodies

AB117813

Anti-STAM1 antibody

Immunoprecipitation - Anti-STAM1 antibody (AB117813)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB151545

Anti-STAM2 antibody [EPR8688]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-STAM2 antibody [EPR8688] (AB151545)

  • 0
  • 0
Primary Antibodies

AB155539

Anti-HGS antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody - C-terminal (AB155539)

  • 4
  • 3
Primary Antibodies

AB189995

Anti-AP-2 complex subunit alpha-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP-2 complex subunit alpha-1 antibody (AB189995)

  • 5
  • 1
Primary Antibodies

AB76004

Anti-EPS15R antibody [EP1146Y]

RabMAb|Recombinant

Western blot - Anti-EPS15R antibody [EP1146Y] (AB76004)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

STAM1 also known as Signal Transducing Adaptor Molecule 1 functions mechanically as an important player in intracellular signal transduction. It operates as an adaptor protein that interacts with various molecules within the cell. STAM1 has a molecular mass of approximately 58 kDa. Researchers observe high expression of STAM1 in the cytoplasmic region of cells being particularly abundant in immune cells. It engages in various protein-protein interactions to exert its function.
Biological function summary

STAM1 participates in endocytic trafficking and cytokine receptor endocytosis. It often associates with the ESCRT-0 (Endosomal Sorting Complex Required for Transport) complex which plays an essential role in sorting ubiquitinated cargo for lysosomal degradation. This protein helps regulate the cellular response to certain cytokines by facilitating the downregulation of activated receptors thereby contributing to signal attenuation.

Pathways

STAM1 involvement can be seen in cytokine signaling pathways and the ubiquitin-proteasome system. In the context of cytokine signaling STAM1 interacts with components like the Janus Kinase (JAK) family and Signal Transducers and Activators of Transcription (STATs) modulating cell growth and immune responses. It also has a significant function in the ubiquitin-dependent endocytic route coordinating with other ESCRT components like HRS (hepatocyte growth factor-regulated tyrosine kinase substrate).

Research links STAM1 with oncogenesis and neurodegenerative conditions. In cancer aberrant STAM1 expression correlates with altered growth factor receptor degradation potentially affecting tumor progression. STAM1's interaction with key proteins like c-MET a receptor tyrosine kinase can influence oncogenic signaling. In neurodegenerative disorders such as Alzheimer's disease STAM1's role in protein trafficking and degradation may contribute to the accumulation of misfolded proteins emphasizing its importance in maintaining cellular homeostasis.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.. (Microbial infection) Plays an important role in Dengue virus entry.
See full target information STAM
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 40:108 PubMed39630363

2024

The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Huang,Xuewei Lao,Chengyan He,Jia Wang,Ying Pan

Frontiers in oncology 12:795971 PubMed35814368

2022

Expression Promotes Lung Adenocarcinoma Progression and Is Associated With Poor Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Jiang,Xiaolu Huang,Xiang Yang,Huixin Zhou,Yumin Wang

Frontiers in oncology 11:611081 PubMed33959493

2021

STAM Prolongs Clear Cell Renal Cell Carcinoma Patients' Survival Inhibiting Cell Growth and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Tuo Deng,Zihao He,Xiaolu Duan,Di Gu,Chao Cai,Wenqi Wu,Yongda Liu,Guohua Zeng
View all publications
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