Anti-ST8SIA5 抗体 (ab184777)
Key features and details
- Rabbit polyclonal to ST8SIA5
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ST8SIA5 antibody -
製品の詳細
Rabbit polyclonal to ST8SIA5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human ST8SIA5 aa 300 to the C-terminus.
Database link: O15466 -
ポジティブ･コントロール
- IHC-P: Human cerebral cortex tissue; ICC: RH-30 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184777の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively. -
組織特異性
Expressed in fetal and adult brain, adult heart and skeletal muscle. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 29 family. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 29906 Human
- Entrez Gene: 225742 Mouse
- Entrez Gene: 364901 Rat
- SwissProt: O15466 Human
- SwissProt: P70126 Mouse
- Unigene: 465025 Human
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別名
- 8-sialyltransferase 8E antibody
- Alpha-2 antibody
- SIA8E_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (ab184777)
Immunohistochemical analysis of human testis tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in seminiferous ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST8SIA5 antibody (ab184777)Immunohistochemical analysis of human cerebral cortex tissue labelled for ST8SIA5 using ab184777. Positive cytoplasmic staining observed in neurons.
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Immunocytochemistry/ Immunofluorescence - Anti-ST8SIA5 antibody (ab184777)Immunofluorescence analysis of RH-30 (human bone marrow metastasis cell line) whole cells labeling ST8SIA5 in the plasma membrane & midbody with ab184777 at 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184777 は論文での使用が確認できていません。