Anti-ST14 抗体 - Catalytic domain (ab28266)
Key features and details
- Rabbit polyclonal to ST14 - Catalytic domain
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ST14 antibody - Catalytic domain
ST14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ST14 - Catalytic domain -
由来種
Rabbit -
特異性
This antibody does not recognize matriptase 2 (MTSP2). -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide based on the catalytic domain of Human ST14.
Immunogen の所有権に関して (Peptide available as ab41264.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28266の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site. -
関連疾患
Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 4 LDL-receptor class A domains.
Contains 1 peptidase S1 domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6768 Human
- Omim: 606797 Human
- SwissProt: Q9Y5Y6 Human
- Unigene: 504315 Human
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別名
- Epithin antibody
- HAI antibody
- Matriptase antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ST14 antibody - Catalytic domain (ab28266)ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab28266 は 5 報の論文で使用されています。
- Marchelletta RR et al. T cell protein tyrosine phosphatase protects intestinal barrier function by restricting epithelial tight junction remodeling. J Clin Invest 131:N/A (2021). PubMed: 34623320
- Sun P et al. Regulation of matriptase and HAI-1 system, a novel therapeutic target in human endometrial cancer cells. Oncotarget 9:12682-12694 (2018). PubMed: 29560101
- Kubyshkin AV et al. THE IMPACT OF ALCOHOL ON PRO-METASTATIC N-GLYCOSYLATION IN PROSTATE CANCER. Krim Z Eksp Klin Med 8:11-20 (2018). PubMed: 31131224
- Sun P et al. Decreasing the ratio of matriptase/HAI-1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer. Mol Med Rep 14:1465-74 (2016). WB ; Human . PubMed: 27356668
- Takeichi T et al. Whole-exome sequencing diagnosis of two autosomal recessive disorders in one family. Br J Dermatol 172:1407-11 (2015). PubMed: 25308318