AB229054

Anti-Ssu72 抗体

Anti-Ssu72 antibody

JP Deleterious:医薬用外劇物

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(1 Publication)

Rabbit Polyclonal SSU72 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SSU72.

別名を表示する

HSPC182, PNAS-120, SSU72, RNA polymerase II subunit A C-terminal domain phosphatase SSU72, CTD phosphatase SSU72

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

Paraffin-embedded rat liver tissue stained for Ssu72 with ab229054 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Ssu72 antibody (AB229054)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ssu72 antibody (ab229054) at 1/1000 dilution

All lanes:

Mouse placenta tissue extract at 50 µg

Predicted band size: 23 kDa

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Supplier Data

Western blot - Anti-Ssu72 antibody (AB229054)

12% SDS-PAGE

All lanes:

Western blot - Anti-Ssu72 antibody (ab229054) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 23 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human SSU72. The exact immunogen used to generate this antibody is proprietary information.

Q9NP77

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ssu72 also known as SSU72 homolog RNA polymerase II CTD phosphatase is an enzyme with a mass of approximately 24 kDa. It functions as a specific phosphatase that targets the C-terminal domain (CTD) of RNA polymerase II. Ssu72 performs its mechanical role by removing phosphate groups from serine 5 residues within the heptad repeats of the CTD which is important for the transcription cycle. The expression of Ssu72 occurs in the nucleus where its activity aligns with its role in transcription regulation.

Biological function summary

The enzymatic function of Ssu72 contributes to gene expression regulation. It plays a significant role during the transition between the transcription initiation and elongation phases. Ssu72 is an integral part of the transcription machinery and is known to associate with the cleavage and polyadenylation complex highlighting its involvement in the pre-mRNA processing. Additionally Ssu72's phosphatase activity helps regulate the interaction between RNA polymerase II and multiple transcription factors and complexes emphasizing its role in maintaining efficient transcription and RNA processing dynamics.

Pathways

Ssu72 functions within the transcription regulation pathway where it interacts with other critical transcription-related proteins such as the Symplekin and TFIIB. Its involvement in the regulation of RNA polymerase II activity connects it to RNA processing pathways including mRNA capping splicing and termination processes. These interactions facilitate the precise control of gene expression by modulating transcription elongation and mRNA processing events ensuring coordinated transcriptional responses.

The dysfunction of Ssu72 can lead to transcriptional dysregulation contributing to cancer and other transcription-associated disorders. Disruptions in its phosphatase activity may have downstream effects on RNA polymerase II and the associated transcription regulation processes. Ssu72's connection to cancer is further supported by its interaction with proteins like PinX1 a negative regulator of telomerase where altered expression or activity could influence cellular proliferation and stability potentially driving oncogenesis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.

See full target information SSU72

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience bulletin 40:421-438 PubMed37864744

2023

Inflammatory Factor IL1α Induces Aberrant Astrocyte Proliferation in Spinal Cord Injury Through the Grin2c/Ca/CaMK2b Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xia,Lu Ding,Changlin Zhang,Qi Xu,Ming Shi,Tianshun Gao,Feng-Quan Zhou,David Y B Deng

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-Ssu72 抗体

Anti-Ssu72 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ssu72 antibody (AB229054)

Western blot - Anti-Ssu72 antibody (AB229054)

Western blot - Anti-Ssu72 antibody (AB229054)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise