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Anti-SSEA1 抗体 [MC-480] (ab16285)

Reviews (9)Q&A (4)References (62)
Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)
  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)

Key features and details

  • Mouse monoclonal [MC-480] to SSEA1
  • Suitable for: IHC-FoFr, Flow Cyt, IHC-P, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-SSEA1 antibody [MC-480]

  • 製品の詳細

    Mouse monoclonal [MC-480] to SSEA1

  • 由来種

    Mouse

  • 特異性

    We guarantee IHC-P in Human only

  • アプリケーション

    適用あり: IHC-FoFr, Flow Cyt, IHC-P, ICCmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. ICC: Mouse embryonic stem cells. Flow Cyt: Mouse ES cells

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab16285の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt (2)
Use at an assay dependent concentration.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/1000.

Fixation with 100% MeOH (5 min) or 4% PFA (10 min).
特記事項
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC
1/1000.

Fixation with 100% MeOH (5 min) or 4% PFA (10 min).

ターゲット情報

  • 関連性

    Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.

画像

  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)

    ab16285 staining SSEA1 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16285 at a 1/1000 dilution and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSEA1 antibody [MC-480] (ab16285)

    IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)
    Flow Cytometry - Anti-SSEA1 antibody [MC-480] (ab16285)This image is taken from an anonymous abreview.
    Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

    See Abreview

  • Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)
    Immunocytochemistry - Anti-SSEA1 antibody [MC-480] (ab16285)This image is courtesy of the laboratory of Peter Andrews.
    The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.

参考文献 (62)

ab16285 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab16285 は 62 報の論文で使用されています。

  • Cui G  et al. WD repeat domain 82 (Wdr82) facilitates mouse iPSCs generation by interfering mitochondrial oxidative phosphorylation and glycolysis. Cell Mol Life Sci 80:218 (2023). PubMed: 37470863
  • Yoshimoto Y  et al. Tenogenic Induction From Induced Pluripotent Stem Cells Unveils the Trajectory Towards Tenocyte Differentiation. Front Cell Dev Biol 10:780038 (2022). PubMed: 35372337
  • Ren Y  et al. Knockout of integrin β1 in induced pluripotent stem cells accelerates skin-wound healing by promoting cell migration in extracellular matrix. Stem Cell Res Ther 13:389 (2022). PubMed: 35908001
  • Ma B  et al. Patient-specific and gene-corrected induced pluripotent stem cell-derived endothelial cells elucidate single-cell phenotype of pulmonary veno-occlusive disease. Stem Cell Reports 17:2674-2689 (2022). PubMed: 36400028
  • Hsieh HL  et al. Induced pluripotent stem cells can improve thrombolytic effect of low-dose rt-PA after acute carotid thrombosis in rat. Stem Cell Res Ther 12:549 (2021). PubMed: 34674761
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 13 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA1 antibody [MC-480]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human iPSC colonies)
Permeabilization
Yes - 0.25% Triton X-100
Specification
human iPSC colonies
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 15 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA1 antibody [MC-480]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (Chicken ESC)
Permeabilization
No
Specification
Chicken ESC
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 06 2016

Immunocytochemistry abreview for Anti-SSEA1 antibody [MC480]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (embryonic)
Specification
embryonic
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% tritone X 100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Salma Sana

Verified customer

投稿 Jan 16 2012

Flow Cytometry abreview for Anti-SSEA1 antibody [MC480]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (embryonic stem cell (D3))
Specification
embryonic stem cell (D3)
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation
Formaldehyde
Permeabilization
No
Gating Strategy
live cells
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 24 2011

Immunohistochemistry - Free Floating abreview for Anti-SSEA1 antibody [MC480]

 Good
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Coronal Brain Sections)
Specification
Coronal Brain Sections
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 11 2011

Flow Cytometry abreview for Anti-SSEA1 antibody [MC480]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (mouse ES cell line D3)
Specification
mouse ES cell line D3
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 03 2011

Question

Thank you for your answer The application I will use is the immunofluorescence on PFA fixed cells  

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Answer

Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.  

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Question

Dear Technical Support I have questions about anti CD15 antibodies. CD15 is synonymous with SSEA-1 but you have to different set of antibodies: CD15 (ab119844 or ab754) and SSEA-1 (ab16285). Do they recognize the same protein? I need Ab against human protein, does the ab16285 react with human? If not may I buy a CD15 antibody to stain the same cells? Which is better for IFr application, the ab119844 or ab754 Thank ou very much  

Read More
Answer

Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some antobodies have the CD15 others the SSEA1 as target name. ab754 and ab119844 recognize human protein and both are suitable for immunohistochemistry on formalin-fixed paraffin-embedded (IHC-P) tissue sections. However, ab16285 only detects murine SSEA1. I am not sure what you mean by IFr application? Is it immunofluorescence (ICC/IF) or immunohistochemistry on frozen tissue (IHC-Fr) section? Could you please specify the sample types you wish to use so that I could recommend the most suitable product for you? I hope this helps and if I can assist further, please do not hesitate to contact me.  

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Question

Hello,I need MSDS for the following products ab19857 and ab16285. Can you kindly email them to me.Thank YouRegards,

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Answer

Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer". I hope this information is useful. Please do not hesitate to contact us again should you have any additional question.

Read More

Question

Dear Tech Support Team,  Our customer is willing to test ab16285 in IHC-Fr with mouse sections. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)? Thanks in advance for your assistance and reply. Have a nice week,

Read More
Answer

DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHc-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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1-10 of 13 Abreviews or Q&A

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