Anti-SSBP1 抗体 (ab74710)
Key features and details
- Rabbit polyclonal to SSBP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SSBP1 antibody
SSBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SSBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
A synthesized peptide derived from internal sequence of human SSBP1.
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ポジティブ･コントロール
- Human pancreas tissue; extracts from HeLa cells and HUVEC cells; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. -
配列類似性
Contains 1 SSB domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 6742 Human
- Entrez Gene: 381760 Mouse
- Entrez Gene: 54304 Rat
- Omim: 600439 Human
- SwissProt: Q04837 Human
- SwissProt: Q9CYR0 Mouse
- SwissProt: P28042 Rat
- Unigene: 490394 Human
see all -
別名
- mitochondrial antibody
- Mt SSB antibody
- Mt-SSB antibody
see all
画像
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Western blot - Anti-SSBP1 antibody (ab74710)All lanes : Anti-SSBP1 antibody (ab74710) at 1/500 dilution
Lane 1 : Extracts from HeLa cells with no peptide
Lane 2 : Extracts from HUVEC cells with no peptide
Lane 3 : Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Additional bands at: 26 kDa, 45 kDa. We are unsure as to the identity of these extra bands.Secondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP1 antibody (ab74710)Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ab74710 at a dilution of 1/50. Left-hand panel: no peptide; right-hand panel: with peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-SSBP1 antibody (ab74710)Immunofluorescence analysis of A549 cells, using ab74710 at a dilution of 1/500. Left-hand panel: no peptide; right-hand panel: with peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab74710 は 3 報の論文で使用されています。
- Reyes A et al. RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA. Front Genet 10:1393 (2019). PubMed: 32082360
- Li Q et al. A functional polymorphism ofSSBP1gene predicts prognosis and response to chemotherapy in resected gastric cancer patients. Oncotarget 8:110861-110876 (2017). PubMed: 29340022
- van Loon B & Samson LD Alkyladenine DNA glycosylase (AAG) localizes to mitochondria and interacts with mitochondrial single-stranded binding protein (mtSSB). DNA Repair (Amst) 12:177-87 (2013). PubMed: 23290262