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リコンビナントRabMAb

Anti-SRP54 抗体 [EPR10851(B)] (ab154796)

Reviews (2) Submit a question References (1)
Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
  • Anti-SRP54 antibody [EPR10851(B)] (ab154796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10851(B)] to SRP54
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SRP54 antibody [EPR10851(B)]
    SRP54 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10851(B)] to SRP54

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human SRP54. The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Human placenta, fetal brain, 293T, Jurkat and HeLa lysates; Human brain and breast carcinoma tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10851(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154796の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

画像

  • Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    All lanes : Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution

    Lane 1 : Human placenta lysate
    Lane 2 : fetal brain lysate
    Lane 3 : 293T lysate
    Lane 4 : Jurkat lysate
    Lane 5 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)

    Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SRP54 antibody [EPR10851(B)] (ab154796)
    Anti-SRP54 antibody [EPR10851(B)] (ab154796)

参考文献 (1)

ab154796 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab154796 は 1 報の論文で使用されています。

  • Zhao Y  et al. Intragenic Transcriptional cis-Antagonism Across SLC6A3. Mol Neurobiol 56:4051-4060 (2019). PubMed: 30259411

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

ChIP abreview for Anti-SRP54 antibody [EPR10851(B)]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Rat Cell lysate - whole cell (BRAIN)
Specification
BRAIN
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 30 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-SRP54 antibody [EPR10851(B)]

 Poor
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Feb 21 2013

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