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リコンビナントRabMAb

Anti-SRGAP1 抗体 [EPR8760(2)] (ab171938)

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Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8760(2)] to SRGAP1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SRGAP1 antibody [EPR8760(2)]
    SRGAP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8760(2)] to SRGAP1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q7Z6B7

  • ポジティブ･コントロール

    • Human fetal brain, Human fetal lung, Human fetal kidney tissue lysates, A549 cells, 293T cell line lysate, Human colon tissue, Human lung tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab171938の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.

  • 組織特異性

    Expressed in brain, lung, kidney, and testis.

  • 配列類似性

    Contains 1 FCH domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SH3 domain.

  • ドメイン

    The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 4930572H05Rik antibody
    • ARHGAP13 antibody
    • FLJ22166 antibody
    • KIAA1304 antibody
    • RGD1566260 antibody
    • Rho GTPase activating protein 13 antibody
    • Rho GTPase-activating protein 13 antibody
    • SLIT ROBO Rho GTPase activating protein 1 antibody
    • SLIT-ROBO Rho GTPase-activating protein 1 antibody
    • SRGAP 1 antibody
    • srGAP1 antibody
    • SRGP1_HUMAN antibody
    see all

画像

  • Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : Human fetal lung tissue lysate
    Lane 5 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 124 kDa

  • Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

    Intracellular flow cytometric analysis of permeabilized 293T cellslabeling SRGAP1 with ab171938 at 1/100 (red)or a rabbit IgG (negative) (green). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

    Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

    Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
    Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

参考文献 (0)

ab171938 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab171938 は論文での使用が確認できていません。

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