Anti-SRFBP1 抗体 (ab109598)
Key features and details
- Rabbit polyclonal to SRFBP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SRFBP1 antibody -
製品の詳細
Rabbit polyclonal to SRFBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 379 and 429 of Human SRFBP1 (NP_689759.2).
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ポジティブ･コントロール
- HeLa cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109598の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
May be involved in regulating transcriptional activation of cardiac genes during the aging process. May play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. -
組織特異性
Abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. -
発生段階
Up-regulated in adult heart (at protein level). -
細胞内局在
Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 153443 Human
- Entrez Gene: 67222 Mouse
- Entrez Gene: 100174161 Orangutan
- Entrez Gene: 291469 Rat
- Omim: 610479 Human
- SwissProt: Q8NEF9 Human
- SwissProt: Q9CZ91 Mouse
- SwissProt: Q5NVE2 Orangutan
see all -
別名
- p49 / STRAP antibody
- p49 antibody
- p49/STRAP antibody
see all
画像
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Western blot - Anti-SRFBP1 antibody (ab109598)All lanes : Anti-SRFBP1 antibody (ab109598) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Predicted band size: 49 kDa -
Immunoprecipitation - Anti-SRFBP1 antibody (ab109598)All lanes : SRFBP1 antibody (ab109598) at 1 µg/ml for WB detection
Lane 1 : HeLa cell lysate (IP with 6 µg/ml of SRFBP1 antibody)
Lane 2 : HeLa cell lysate (IP with IgG Control)
Lysates/proteins at 1 mg per lane.
All lanes : Anti-SRFBP1 antibody (ab109598) at 1 µg/ml
Lane 1 : HeLa cell lysate (IP with 6 µg/ml of SRFBP1 antibody)
Lane 2 : HeLa cell lysate (IP with IgG Control)
Lysates/proteins at 1000 µg per lane.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109598 は 1 報の論文で使用されています。
- Kusama K et al. Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers. Life (Basel) 12:N/A (2022). PubMed: 35207596