Anti-SREC-I 抗体 [EPR25615-161] (ab300407)
![Flow Cytometry (Intracellular) - Anti-SREC-I antibody [EPR25615-161] (AB300407)](https://www.abcam.co.jp/ps/products/300/ab300407/Images/AB300407-3-anti-sreci-antibody-epr25615161-flow-cytometry-hek293t.jpg "Flow Cytometry (Intracellular) - Anti-SREC-I antibody [EPR25615-161] (AB300407)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25615-161] to SREC-I
- Suitable for: Flow Cyt, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SREC-I antibody [EPR25615-161]
SREC-I 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR25615-161] to SREC-I -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, Flow Cyt (Intra)more details
適用なし: ICC/IF,IHC-P,IP or WB -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): HEK-293T (human embryonic kidney) transfected with myc-tagged SREC-II expression construct , HEK-293T transfected with myc-tagged SREC-I expression construct. Floy Cyt: HUVEC (human umbilical vein endothelial cells)
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR25615-161 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300407の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/50.
|
|
| Flow Cyt (Intra) |
1/500.
|
| 特記事項 |
|---|
|
Flow Cyt
1/50. |
|
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
Mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Mediates heterophilic interactions, suggesting a function as adhesion protein. Plays a role in the regulation of neurite-like outgrowth. -
組織特異性
Endothelial cells. -
配列類似性
Contains 6 EGF-like domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8578 Human
- Omim: 607873 Human
- SwissProt: Q14162 Human
- Unigene: 647430 Human
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別名
- Acetyl LDL receptor antibody
- Endothelial cells scavenger receptor antibody
- SCARF1 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-SREC-I antibody [EPR25615-161] (AB300407)
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized HEK-293T (human embryonic kidney) transfected with myc-tagged SREC-II expression construct (Left) / HEK-293T transfected with myc-tagged SREC-I expression construct (Right) cells labelling SREC-I with ab300407 at 1/500 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with ab300407, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 647.
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![Flow Cytometry - Anti-SREC-I antibody [EPR25615-161] (AB300407)](https://www.abcam.co.jp/ps/products/300/ab300407/Images/AB300407-2-anti-sreci-antibody-epr25615161-flow-cytometry-huvec.jpg)
Flow Cytometry - Anti-SREC-I antibody [EPR25615-161] (AB300407)Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell, Right) cells labelling SREC-I with ab300407 at 1/50 dilution (1&#g) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control (left): Jurkat (PMID: 23892722). -
![Anti-SREC-I antibody [EPR25615-161] (AB300407)](https://www.abcam.co.jp/ps/products/300/ab300407/Images/AB300407-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-SREC-I antibody [EPR25615-161] (AB300407)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300407 は論文での使用が確認できていません。