AB30682

Anti-SREBP2 抗体

Name: Anti-SREBP2 抗体 (ab30682) | Abcam
Brand: Abcam Limited
SKU: AB30682
Price: 72000 JPY
Availability: InStock
Rating: 4 (12 reviews)

Anti-SREBP2 antibody

(12 Reviews)

(214 Publications)

Anti-SREBP2 antibody (ab30682) is a rabbit polyclonal antibody detecting SREBP2 in Western Blot, IHC-Fr, ICC/IF. Suitable for Human, Rat.

- Over 160 publications
- Trusted since 2006

別名を表示する

BHLHD2, SREBP2, SREBF2, Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, bHLHd2

3 Images

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Western blot - Anti-SREBP2 antibody (AB30682)

Observed bands 126 kDa, 55kDa (cleaved form)

All lanes:

Western blot - Anti-SREBP2 antibody (ab30682) at 1/200 dilution

Lane 1:

Human fibroblast cell lysate at 60 µg

Lane 2:

Rat brown fat homogenate at 60 µg

Lane 3:

Rat testis supernatant at 60 µg

Predicted band size: 123 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

AbReview35456****

Western blot - Anti-SREBP2 antibody (AB30682)

Blocking : 7% milk for 60 minutes at 20^oC.

All lanes:

Western blot - Anti-SREBP2 antibody (ab30682) at 1/1000 dilution

All lanes:

Human retina whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution

Predicted band size: 123 kDa

Observed band size: 126 kDa,55 kDa,60 kDa

true

Exposure time: 6min

This image is courtesy of an Abreview by Dongil Kim.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-Fr, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human SREBF2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q12772

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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製品の詳細

Product Specifications
Anti-SREBP2 antibody (ab30682) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-Fr, WB in chicken, human, rat samples.
Anti-SREBP2 antibody (ab30682) specifically detects SREBP2 (UniProt ID: Q12772; Molecular weight: 124kDa) and is sold in 250 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-SREBP2 antibody (ab30682) has high sensitivity and specificity.
Anti-SREBP2 antibody (ab30682) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.
Anti-SREBP2 antibody (ab30682) has 11 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Sterol regulatory element-binding protein 2. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed : 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed : 32322062).. Processed sterol regulatory element-binding protein 2. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed : 12177166, PubMed : 32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed : 12177166, PubMed : 7903453). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed : 12177166, PubMed : 32322062).

See full target information SREBF2

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文献 (214)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e71051 PubMed40955587

2025

NONO Maintains SREBP-Regulated Cholesterol Biosynthesis via RNA Binding in Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Song Zhang,Hayley Ingram,Jack Cooper,Alina Naveed,Stefan G Kathman,Garrett L Lindsey,Tao Liu,Charles S Bond,Jamie I Fletcher,Benjamin F Cravatt,Archa H Fox

Scientific reports 15:23103 PubMed40595126

2025

SREBP-2 promotes cancer progression through the mevalonate-Akt pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie You,Lili Su,Shuping Weng,Jing Li,Xingguang Wang,Bin Liang,Daowei Li,Lijun Li,Haiquan Chen

Cancer communications (London, England) 45:1038-1071 PubMed40443016

2025

Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghan Zhu,Yixiao Xiong,Yuxin Zhang,Huifang Liang,Kun Cheng,Yuanxiang Lu,Guangzhen Cai,Yang Wu,Yunhui Fan,Xiaoping Chen,Hong Zhu,Zeyang Ding,Wanguang Zhang

European journal of medical research 30:296 PubMed40247356

2025

Mammalian Ste20-like kinase 1 regulates AMPK to mitigate the progression of non-alcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Wang,Chenglei Zhang,Jie Ma,Jiarui Li,Yuanyuan Wu,Yanru Ren,Jianning Li,Yan Li,Yi Yang

Cell death & disease 16:308 PubMed40240362

2025

IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Anbo Gao,Juan Zou,Tian Zeng,Mei Qin,Xing Tang,Ting Yi,Guangming Song,Jie Zhong,Yuhuan Zeng,Wenchao Zhou,Qin Gao,Qunfeng Zhang,Juan Zhang,Yukun Li

International journal of chronic obstructive pulmonary disease 20:411-423 PubMed40008109

2025

Cholesterol Accumulation Enhances Cigarette Smoke-Induced Airway Epithelial Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Du Jing,Jin-Kang Yu,Hai-Pin Chen,Ling-Ling Dong,Wen Li,Zhou-Yang Li,Jie-Sen Zhou

The Journal of clinical investigation 135: PubMed39998898

2025

An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangioma.

Applications

Unspecified application

Species

Unspecified reactive species

Annegret Holm,Matthew S Graus,Jill Wylie-Sears,Jerry Wei Heng Tan,Maya Alvarez-Harmon,Luke Borgelt,Sana Nasim,Long Chung,Ashish Jain,Mingwei Sun,Liang Sun,Pascal Brouillard,Ramrada Lekwuttikarn,Yanfei Qi,Joyce Teng,Miikka Vikkula,Harry Kozakewich,John B Mulliken,Mathias Francois,Joyce Bischoff

Nature communications 16:1241 PubMed39890808

2025

Copy number amplification of FLAD1 promotes the progression of triple-negative breast cancer through lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qing Song,Tian-Jian Yu,Yang Ou-Yang,Jia-Han Ding,Yi-Zhou Jiang,Zhi-Ming Shao,Yi Xiao

Experimental and therapeutic medicine 29:46 PubMed39885913

2025

Lindl. alkaloids improve lipid metabolism by increasing LDL uptake through regulation of the LXRα/IDOL/LDLR pathway and inhibition of PCSK9 expression in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Sun,Hao-Rui Liu,Ya-Xin Zhu,Wei Zhang,Jing-Shan Shi,Qin Wu,Rui-Xia Xu

Cancer cell international 25:25 PubMed39871246

2025

DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Shen Luo,Yue Yin,Yang Liu,Xiaomei Sun,Ling Qiu,Xin Wu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SREBP2 抗体

Anti-SREBP2 antibody

Western blot - Anti-SREBP2 antibody (AB30682)

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

Western blot - Anti-SREBP2 antibody (AB30682)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (214)

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組合せ製品

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise