Anti-SREBP1 抗体 [2A4] (ab3259)
Key features and details
- Mouse monoclonal [2A4] to SREBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-SREBP1 antibody [2A4]
SREBP1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A4] to SREBP1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
エピトープ
Amino acids 301 - 407. -
ポジティブ・コントロール
- WB: HeLa and HAP1 whole cell lysates.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
2A4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3259の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (11) |
Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa).
By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). Based on in-house testing, Abcam recommends using a 5% milk block for this product. |
特記事項 |
---|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa). By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). Based on in-house testing, Abcam recommends using a 5% milk block for this product. |
ターゲット情報
-
機能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). -
組織特異性
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. -
配列類似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. -
細胞内局在
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
-
参照データベース
- Entrez Gene: 6720 Human
- Omim: 184756 Human
- SwissProt: P36956 Human
- Unigene: 592123 Human
-
別名
- ADD 1 antibody
- bHLHd1 antibody
- Class D basic helix-loop-helix protein 1 antibody
see all
画像
-
All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HAP1 whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible cleavage fragment)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (104)
ab3259 は 104 報の論文で使用されています。
- Gao W et al. Inositol-requiring enzyme 1α and c-Jun N-terminal kinase axis activation contributes to intracellular lipid accumulation in calf hepatocytes. J Dairy Sci 107:3127-3139 (2024). PubMed: 37939835
- Yang Y et al. Effects of Boron on Fat Synthesis in Porcine Mammary Epithelial Cells. Biol Trace Elem Res 202:190-198 (2024). PubMed: 37103639
- Li YQ et al. RARRES2 regulates lipid metabolic reprogramming to mediate the development of brain metastasis in triple negative breast cancer. Mil Med Res 10:34 (2023). PubMed: 37491281
- Gu L et al. Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness. Cell Metab 35:1009-1021.e9 (2023). PubMed: 37084733
- Stevanović-Silva J et al. Gestational Exercise Antagonises the Impact of Maternal High-Fat High-Sucrose Diet on Liver Mitochondrial Alterations and Quality Control Signalling in Male Offspring. Int J Environ Res Public Health 20:N/A (2023). PubMed: 36674144