Anti-Src (phospho Y419) 抗体 (ab4816)

Thank you for taking time to complete our questionnaire. I am sorry to hear that the anti-Src (phospho Y418) antibody(ab4816) has not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, there are a few further details I'd like to clarify:

1. What exposure time was used to obtain the image shared with us?

2. Have the samples used in this blot been used to detect any other targets? Eg. loading controls such as actin or tubulin? Or has pan-Src been measured in the samples?

3. Has the secondary antibody been used successfully with any other primary antibodies?

There are a few suggestions I would make in order to help optimize the results from ab4816.

1. I would suggest introducing 0.1% Tween 20 into all the buffers used (blocking, primary and secondary antibody diluents as well as the wash buffer). This can help to reduce non-specific binding of the membrane.

2. I would also suggest blocking the membrane at room temperature for ˜1-2 hours then incubate the primary antibody overnight at 4 degrees with aggitation. This can improve the specificity observed with antibodies.

3. I would also suggest performing a "no primary control" in order to find out how much the secondary antibody is contributing to the non-specificity observed.

4. It may also be worthwhile to reduce the amount of protein loaded on the gel. I would try 10 and 25 ug/well to see how this may improve the signals observed.

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab4816 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I just received some good news. I was able to obtain the specific concentration for 2 of our current lots, which are: lot 442382, 0.4 mg/mL lot 502322, 0.5 mg/mL

Thank you for your enquiry. I have performed a sequence alignment of the immunogen sequence and I can tell you that the corresponding C.elegans amino acid sequence has 8 out of 14 amino acid residues in common. However it is most dissimilar in the region immediately surrounding the phosphorylated tyrosine. Based upon this, it is very difficult to predict whether or not the antibody will react with C.elegans Src. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your patience. The batches of ab4816 and ab4817 that we currently have in stock are at a concentration of 0.25 mg/mL. If you have any more questions, please contact us again.