Anti-Src (phospho S75) 抗体 (ab79308)

I am very pleased to hear you would like to accept our offer these antibodiesindog. This code will give you: 1 freeprimary antibodybefore the expiration date for each antibody tested. To redeem this offer, please submit an Abreview for dog for each of the antibodies tested and include the specific code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for your reply and the additional information which is very helpful. The fact that the total protein gives you the same band pattern might indicate that these bands could be real for your rat samples. Which antibody did you use to detect total Src? Rat is listed as "predicted to work with" on the datasheet which means that it is only predicted based on sequence homology for the immunogen. We have no data for rat samples so far and do not know if and how well it would work with this species. Does the treatment to inactivate Src give you the expected results (increase/decrease in signal)? The total protein should nevertheless remain unchanged as the total protein should not be affected by changes in phosphorylation. Do you see this as well? I'd be happy to issue you a testing discount retroactively since you are using the antibody on an untested species. The discount code will give you a free antibody on a future purchase after the rat data have been submitted as an Abreview. Here are more details on how this works: For UNTESTED species and/or applications, we have established a testing discount program. The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount. Please let me know if I shall send you a discount code. I look forward to hear back from you.

Thank you for contacting us and for including the WB image. The band shown on our datasheet runs at about 55 to 60 kDa and was blocked with the immunizing peptide. Only human samples have so far been tested with this antibody. This antibody detects phospho-Src. What does your total protein sample look like? And have you tried any other sample (e.g. human sample or cell line)? What antibody dilution are you using? And what are the blocking conditions? I am not quite certain what the 85 kDa band could be. The protein can be potentially phosphorylated at multiple sites, and depending on the location of the phospho-site (e.g. in proximity to a Proline residue) the MW can sometimes shift to a higher MW than expected. But since we have not tried rat samples, I am not sure if this band pattern is sample specific or species specific or if the antibody gives extra (non-specific?) bands in rat samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.