JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232847

Anti-SPTLC1 抗体

Anti-SPTLC1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SPTLC1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Mouse, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Sptlc1 aa 100 to C-terminus.

別名を表示する

Lcb1, Sptlc1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, LCB 1, SPT 1, SPT1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)

Formalin-fixed, paraffin-embedded mouse uterus tissue stained for SPTLC1 using ab232847 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)

Formalin-fixed, paraffin-embedded mouse liver tissue stained for SPTLC1 using ab232847 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC1 antibody (AB232847)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for SPTLC1 using ab232847 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-SPTLC1 antibody (AB232847)
  • WB

Supplier Data

Western blot - Anti-SPTLC1 antibody (AB232847)

All lanes:

Western blot - Anti-SPTLC1 antibody (ab232847) at 1 µg/mL

All lanes:

Recombinant mouse SPTLC1 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

false

Western blot - Anti-SPTLC1 antibody (AB232847)
  • WB

Supplier Data

Western blot - Anti-SPTLC1 antibody (AB232847)

All lanes:

Western blot - Anti-SPTLC1 antibody (ab232847) at 1 µg/mL

Lane 1:

Mouse liver lysate

Lane 2:

Mouse lung lysate

Lane 3:

Mouse brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Mouse Sptlc1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O35704

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
バッファー組成
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed : 28100772). The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (By similarity). Required for adipocyte cell viability and metabolic homeostasis (PubMed : 28100772).
See full target information Sptlc1
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2218-2233 PubMed40490489

2025

Dark-phase melatonin administration does not reduce blood pressure but induces changes in parameters related to the control of the cardiovascular system in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Mauer Sutovska,Lubos Molcan,Peter Stefanik,Michal Zeman

Journal of cellular biochemistry 121:1552-1562 PubMed31512789

2019

Decreased SPTLC1 expression predicts worse outcomes in ccRCC patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Kai Zhu,Wen-Hao Xu,Jun Wang,Yong-Qiang Huang,Mierxiati Abudurexiti,Yuan-Yuan Qu,Yi-Ping Zhu,Hai-Liang Zhang,Ding-Wei Ye
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB176706

Anti-SPTLC1 antibody

Immunoprecipitation - Anti-SPTLC1 antibody (AB176706)

  • 0
  • 0

組合せ製品

Assay Kits

AB133118

Sphingomyelin Assay Kit

Functional Studies - Sphingomyelin Assay Kit (AB133118)

  • 5
  • 1
Primary Antibodies

AB215191

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)

  • 5
  • 4
Primary Antibodies

AB307433

Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Serine Palmitoyltransferase antibody [EPR26954-39] - BSA and Azide free (AB307433)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB93015

Anti-AIM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIM2 antibody (AB93015)

  • 4
  • 8

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com