Anti-Spt6 抗体 (ab32820)
Key features and details
- Rabbit polyclonal to Spt6
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Spt6 antibody -
製品の詳細
Rabbit polyclonal to Spt6 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Zebrafish
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免疫原
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ポジティブ･コントロール
- This antibody gave a positive signal for the following cell lysates: HeLa Nuclear; HeLa; Jurkat.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB | (1) |
1/500. Detects a band of approximately 230 kDa (predicted molecular weight: 199 kDa).
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
1/500. Detects a band of approximately 230 kDa (predicted molecular weight: 199 kDa). |
ターゲット情報
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機能
Acts to stimulate transcriptional elongation by RNA polymerase II. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SPT6 family.
Contains 1 S1 motif domain.
Contains 1 SH2 domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6830 Human
- Entrez Gene: 20926 Mouse
- Entrez Gene: 337866 Zebrafish
- Omim: 601333 Human
- SwissProt: Q7KZ85 Human
- SwissProt: Q3TY72 Mouse
- SwissProt: Q62383 Mouse
- SwissProt: Q8UVK2 Zebrafish
see all -
別名
- emb 5 antibody
- hSPT6 antibody
- KIAA0162 antibody
see all
画像
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Western blot - Anti-Spt6 antibody (ab32820)Lane 1 : Marker
Lanes 2-4 : Anti-Spt6 antibody (ab32820) at 1/500 dilution
Lane 5 : High Mark marker
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg
Secondary
Lanes 2-4 : IR Dye 680 Conjugated Goat anti Rabbit IgG (H+L) at 1/21250 dilution
Performed under reducing conditions.
Predicted band size: 199 kDa
Observed band size: 230 kDa why is the actual band size different from the predicted?
Ab32820 detects a band at approximately 230kDa. Other antibodies to this target detect a band in this region and it is understood that this band corresponds to the native Spt6 protein. -
Immunoprecipitation - Anti-Spt6 antibody (ab32820)Spt6 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Spt6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32820.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 230kDa: Spt6
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab32820 は 11 報の論文で使用されています。
- Bobkov GOM et al. Spt6 is a maintenance factor for centromeric CENP-A. Nat Commun 11:2919 (2020). PubMed: 32522980
- Obara EAA et al. SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells. Nat Commun 11:4709 (2020). PubMed: 32948765
- Dembowski JA et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog 13:e1006166 (2017). PubMed: 28095497
- Soboleva TA et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . PubMed: 28234895
- Gérard A et al. The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells. Cell Host Microbe 17:107-17 (2015). PubMed: 25590759