Anti-Spry-2 抗体 (ab85670)
Key features and details
- Rabbit polyclonal to Spry-2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Spry-2 antibody
Spry-2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Spry-2 -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab180527.
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アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human Spry-2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101081) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following lysates: Human Brain Tissue; Human Lung Tissue; Human Heart Tissue; Jurkat Whole Cell Lysate; Caco2 Whole Cell Lysate; SW480 Whole Cell Lysate. IHC-P: Human skin melanoma FFPE tissue sections
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85670の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
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ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. -
配列類似性
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
ドメイン
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF. - Information by UniProt
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参照データベース
- Entrez Gene: 10253 Human
- Entrez Gene: 24064 Mouse
- Entrez Gene: 100173576 Orangutan
- Entrez Gene: 306141 Rat
- Omim: 602466 Human
- SwissProt: O43597 Human
- SwissProt: Q9QXV8 Mouse
- SwissProt: Q5R959 Orangutan
see all -
別名
- hSPRY 2 antibody
- hSPRY2 antibody
- MGC23039 antibody
see all
画像
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Western blot - Anti-Spry-2 antibody (ab85670)All lanes : Anti-Spry-2 antibody (ab85670) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 100 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody (ab85670)ICC/IF image of ab85670 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85670, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 10µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody (ab85670)IHC image of Spry-2 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85670, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab85670 は 10 報の論文で使用されています。
- Meng L et al. MeCP2 inhibits ischemic neuronal injury by enhancing methylation of the FOXO3a promoter to repress the SPRY2-ZEB1 axis. Exp Mol Med 54:1076-1085 (2022). PubMed: 35915222
- Su W et al. ARAF protein kinase activates RAS by antagonizing its binding to RASGAP NF1. Mol Cell 82:2443-2457.e7 (2022). PubMed: 35613620
- Zhang P et al. K63-linked ubiquitination of DYRK1A by TRAF2 alleviates Sprouty 2-mediated degradation of EGFR. Cell Death Dis 12:608 (2021). PubMed: 34117217
- Che F et al. Increased expression of miR-27 predicts poor prognosis and promotes tumorigenesis in human multiple myeloma. Biosci Rep 39:N/A (2019). PubMed: 30837325
- Liao W et al. BMSC-derived exosomes carrying microRNA-122-5p promote proliferation of osteoblasts in osteonecrosis of the femoral head. Clin Sci (Lond) 133:1955-1975 (2019). PubMed: 31387936
- Holter MC et al. The Noonan Syndrome-linked Raf1L613V mutation drives increased glial number in the mouse cortex and enhanced learning. PLoS Genet 15:e1008108 (2019). PubMed: 31017896
- Li Y et al. microRNA-23a promotes cell growth and metastasis in gastric cancer via targeting SPRY2-mediated ERK signaling. Oncol Lett 15:8433-8441 (2018). PubMed: 29805579
- Hagiwara M & Nakase I Epidermal growth factor induced macropinocytosis directs branch formation of lung epithelial cells. Biochem Biophys Res Commun 507:297-303 (2018). PubMed: 30442367
- Hagiwara M et al. In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells. Tissue Eng Part C Methods 23:323-332 (2017). IHC-P ; Human . PubMed: 28471293
- Bachofner M et al. Large-Scale Quantitative Proteomics Identifies the Ubiquitin Ligase Nedd4-1 as an Essential Regulator of Liver Regeneration. Dev Cell 42:616-625.e8 (2017). PubMed: 28890072