Anti-SPRED2 抗体 (ab153700)
Key features and details
- Rabbit polyclonal to SPRED2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPRED2 antibody -
製品の詳細
Rabbit polyclonal to SPRED2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment, corresponding to a region within acids 79-417 of Human SPRED2 (UniProt: Q7Z698).
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ポジティブ･コントロール
- 293T and A431 whole cell lysates; Human Breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153700の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. -
細胞内局在
Cell Membrane - peripheral membrane protein and Cytoplasmic -
参照データベース
- Entrez Gene: 200734 Human
- Entrez Gene: 114716 Mouse
- Entrez Gene: 305539 Rat
- Omim: 609292 Human
- SwissProt: Q7Z698 Human
- SwissProt: Q924S7 Mouse
- SwissProt: Q3C2P8 Rat
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別名
- C79158 antibody
- FLJ21897 antibody
- FLJ31917 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRED2 antibody (ab153700)Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling SPRED2 with ab153700 at 1/500 dilution.
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Western blot - Anti-SPRED2 antibody (ab153700)Anti-SPRED2 antibody (ab153700) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 48 kDa
10% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab153700 は 8 報の論文で使用されています。
- Zhu L et al. microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2. Histol Histopathol 38:1269-1282 (2023). PubMed: 36704943
- Liu T et al. Sprouty-related proteins with EVH1 domain (SPRED2) prevents high-glucose induced endothelial-mesenchymal transition and endothelial injury by suppressing MAPK activation. Bioengineered 13:13882-13892 (2022). PubMed: 35707829
- Chen D et al. MicroRNA-218 aggravates H2O2-induced damage in PC12 cells via spred2-mediated autophagy. Exp Ther Med 22:1352 (2021). PubMed: 34659498
- Wang X et al. Thrombin aggravates hypoxia/reoxygenation injury of astrocytes by activating the autophagy pathway mediated by SPRED2. Exp Ther Med 22:1107 (2021). PubMed: 34504561
- Hu L et al. LncRNA TUG1 reverses LPS-induced cell apoptosis and inflammation of macrophage via targeting MiR-221-3p/SPRED2 axis. Biosci Biotechnol Biochem 84:2458-2465 (2020). PubMed: 32841583
- Susanto A et al. Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation. Exp Eye Res 178:160-175 (2019). PubMed: 30290165
- Rusan M et al. Suppression of Adaptive Responses to Targeted Cancer Therapy by Transcriptional Repression. Cancer Discov 8:59-73 (2018). PubMed: 29054992
- Zhao G et al. Negative regulation of lens fiber cell differentiation by RTK antagonists Spry and Spred. Exp Eye Res 170:148-159 (2018). WB . PubMed: 29501879