Anti-SPOP 抗体 (ab168619)
Key features and details
- Mouse polyclonal to SPOP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SPOP antibody
SPOP 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to SPOP -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis
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免疫原
Recombinant full length protein within Human SPOP aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- SPOP transfected 293T cell lysate; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab168619の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Inhibits IPF1/PDX1 transactivation of established target promoters, such as insulin, may be by recruiting a repressor complex (By similarity). In complex with CUL3, involved in ubiquitination of BMI1, H2AFY and DAXX, and probably also in ubiquitination and proteasomal degradation of Gli2 or Gli3. -
組織特異性
Widely expressed. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the Tdpoz family.
Contains 1 BTB (POZ) domain.
Contains 1 MATH domain. -
ドメイン
The MATH domain interacts with H2AFY and BMI1. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 530618 Cow
- Entrez Gene: 8405 Human
- Entrez Gene: 20747 Mouse
- Entrez Gene: 779391 Xenopus laevis
- Entrez Gene: 394599 Xenopus tropicalis
- Entrez Gene: 100005514 Zebrafish
- Omim: 602650 Human
- SwissProt: Q0VCW1 Cow
see all -
別名
- BTBD32 antibody
- HIB homolog 1 antibody
- Roadkill homolog 1 antibody
see all
画像
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Western blot - Anti-SPOP antibody (ab168619)All lanes : Anti-SPOP antibody (ab168619) at 1 µg/ml
Lane 1 : SPOP transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes :
Goat Anti-Mouse IgG (H&L)-HRP
at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 42 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SPOP antibody (ab168619)Immunofluorescent analysis of HeLa cells labeling SPOP with ab168619 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab168619 は 3 報の論文で使用されています。
- Gong H et al. APPBP2 enhances non-small cell lung cancer proliferation and invasiveness through regulating PPM1D and SPOP. EBioMedicine 44:138-149 (2019). PubMed: 31105033
- Li Y et al. SPOP Regulates The Biological Mechanism Of Ovarian Cancer Cells Through The Hh Signaling Pathway. Onco Targets Ther 12:9239-9248 (2019). PubMed: 31819473
- Adelaiye-Ogala R et al. Therapeutic Targeting of Sunitinib-Induced AR Phosphorylation in Renal Cell Carcinoma. Cancer Res 78:2886-2896 (2018). PubMed: 29572225
