AB223256

Anti-splicing factor 1 抗体 [EPR22437-50]

Anti-splicing factor 1 antibody [EPR22437-50]

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal splicing factor 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

ZFM1, ZNF162, SF1, Splicing factor 1, Mammalian branch point-binding protein, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, BBP, mBBP

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling splicing factor 1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis (PMID : 25145264; PMID : 20736371) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling splicing factor 1 with ab223256 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).

Secondary antibody only control : Used PBS insread of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling splicing factor 1 with ab223256 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling splicing factor 1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human stomach (PMID : 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Lab

Immunoprecipitation - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Splicing factor 1 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab223256 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab223256 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab223256 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab223256 in HeLa whole cell lysate

Blocking/Dilution buffer : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-splicing factor 1 antibody [EPR22437-50] (ab223256) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab223256 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 68 kDa

Observed band size: 70 kDa,80 kDa

false

Exposure time: 15s

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling splicing factor 1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse testis (PMID : 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling splicing factor 1 with ab223256 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in Neuro-2a cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling splicing factor 1 with ab223256 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID : 25145264) is observed. The section was incubated with ab223256 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Lab

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 8752089; PMID : 17383426; PMID : 10103072; PMID : 25145264).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (ab223256) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 70 kDa,80 kDa

false

Exposure time: 26s

Lab

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.

All lanes:

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (ab223256) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) frozen lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70-80 kDa

false

Exposure time: 7s

Lab

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 8752089; PMID : 17383426; PMID : 10103072; PMID : 25145264).

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (ab223256) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 70 kDa,80 kDa

false

Exposure time: 26s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22437-50

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P, Flow Cyt (Intra), ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This protein is easy to be degraded, to minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting right away.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Splicing factor 1 also known as SF1 or mBBP plays an important role in the splicing of pre-mRNA. It binds to the branch point sequence of intronic regions facilitating the assembly of the spliceosome complex necessary for excising introns and joining exons. The protein has a molecular mass of approximately 75 kDa. SF1 is expressed in a variety of tissues indicating its fundamental role in mRNA processing across different cell types.

Biological function summary

The protein engages in the early stages of spliceosome complex formation working in concert with other splicing factors such as U2AF and SF3b. SF1 is a specific component required in spliceosome assembly contributing to the recognition of correct splice sites. This step is essential for ensuring the accuracy and efficiency of mRNA splicing which is critical for generating mature mRNA transcripts ready for translation. SF1’s involvement with the spliceosome complex itself highlights its importance in cell function regulation.

Pathways

SF1's activity influences the splicing pathway. This pathway a part of the comprehensive gene expression mechanism is essential for regulating cellular processes through mRNA modification. SF1 interacts with proteins like U2AF65 and U2 snRNP vital components of the spliceosome to facilitate splicing. Additionally SF1 indirectly impacts alternative splicing pathways thereby contributing to mRNA diversity and functionality.

Aberrant SF1 function relates to cancer and neurological disorders. Alterations in SF1 expression or mutations can disrupt normal splicing leading to dysregulation of gene expression and pathogenic outcomes. In particular SF1 has been implicated in breast cancer and spinal muscular atrophy. It connects to other proteins such as BRCA1 in cancer and SMN1 in spinal muscular atrophy highlighting how its dysregulation can contribute to disease pathogenesis. Understanding SF1's function and interactions could enhance knowledge of disease mechanisms and potential therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

See full target information SF1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Biology 9: PubMed33202710

2020

Deficiency of Splicing Factor 1 (SF1) Reduces Intestinal Polyp Incidence in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jyotsna D Godavarthi,Shahrazad Polk,Lisa Nunez,Amruthesh Shivachar,Nancy L Glenn Griesinger,Angabin Matin

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-splicing factor 1 抗体 [EPR22437-50]

Anti-splicing factor 1 antibody [EPR22437-50]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Flow Cytometry (Intracellular) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunoprecipitation - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunocytochemistry/ Immunofluorescence - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

Western blot - Anti-splicing factor 1 antibody [EPR22437-50] (AB223256)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise