Anti-sPLA2-X 抗体 [EPR11202] (ab166634)
![Western blot - Anti-sPLA2-X antibody [EPR11202] (ab166634)](https://www.abcam.co.jp/ps/products/166/ab166634/Images/ab166634-164899-anti-phospholipase-a2-x-antibody-epr11202-western-blot.jpg "Western blot - Anti-sPLA2-X antibody [EPR11202] (ab166634)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11202] to sPLA2-X
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-sPLA2-X antibody [EPR11202]
sPLA2-X 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11202] to sPLA2-X -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human sPLA2-X.
Database link: O15496 -
ポジティブ･コントロール
- TT, A549, MCF7 and Caco 2 cell lysates; Human pancreas and tonsil tissues; permeabilized A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11202 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine. -
組織特異性
Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon. -
配列類似性
Belongs to the phospholipase A2 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 8399 Human
- Entrez Gene: 26565 Mouse
- Entrez Gene: 29359 Rat
- Omim: 603603 Human
- SwissProt: O15496 Human
- SwissProt: Q9QXX3 Mouse
- SwissProt: Q9QZT3 Rat
- Unigene: 567366 Human
see all -
別名
- Group 10 secretory phospholipase A2 antibody
- Group X secretory phospholipase A2 antibody
- GX sPLA2 antibody
see all
画像
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Western blot - Anti-sPLA2-X antibody [EPR11202] (ab166634)All lanes : Anti-sPLA2-X antibody [EPR11202] (ab166634) at 1/1000 dilution
Lane 1 : TT cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)](https://www.abcam.co.jp/ps/products/166/ab166634/Images/ab166634-164898-anti-phospholipase-a2-x-antibody-epr11202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)](https://www.abcam.co.jp/ps/products/166/ab166634/Images/ab166634-164897-anti-phospholipase-a2-x-antibody-epr11202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sPLA2-X antibody [EPR11202] (ab166634)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-sPLA2-X antibody [EPR11202] (ab166634)](https://www.abcam.co.jp/ps/products/166/ab166634/Images/ab166634-164896-anti-phospholipase-a2-x-antibody-epr11202-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-sPLA2-X antibody [EPR11202] (ab166634)Intracellular flow cytometric analysis of permeabilized A549 cells using ab166634 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-sPLA2-X antibody [EPR11202] (ab166634)](https://www.abcam.co.jp/ps/products/166/ab166634/Images/ab166634-5-benefits-of-recombinant-antibodies.png)
Anti-sPLA2-X antibody [EPR11202] (ab166634)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab166634 は 3 報の論文で使用されています。
- Tan G et al. PLA2G10 facilitates the cell-cycle progression of soft tissue leiomyosarcoma cells at least by elevating cyclin E1/CDK2 expression. Biochem Biophys Res Commun 527:525-531 (2020). PubMed: 32423798
- Zhang D et al. The inhibitory effect of saPLI?, a snake sourced PLA2 inhibitor on carrageenan-induced inflammation in mice. Toxicon 151:89-95 (2018). PubMed: 30003915
- Nolin JD et al. Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen. JCI Insight 2:N/A (2017). WB ; Mouse . PubMed: 29093264