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リコンビナントRabMAb

Anti-SPHK1 抗体 [EPR24511-37] (BSA and Azide free) (ab302715)

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Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
  • Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24511-37] to SPHK1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free)
    SPHK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24511-37] to SPHK1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Whole cell lysates: Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, Hela transfected with siRNA targeting SPHK1, U-118 MG (human brain glioma cell), SW1353 (human chondrosarcoma fibroblast), HeLa, HepG2 (human hepatocellular carcinoma epithelial cell). Tissue lysates: Human kidney, heart, lung. IP: Whole cell lysates: U-118 MG (human brain glioblastoma cell), HepG2 (human hepatocellular carcinoma epithelial cell).

  • 特記事項

    ab302715 is a carrier free version of ab302714. 

    ab302714 does not react in: WB and immunoprecipitation with mouse and rat species: IHC-P, ICC, and intracellular flow cytometry with human, mouse, and rat species. 

     

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302715の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 42-52 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 42-52 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

  • 組織特異性

    Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.

  • 配列類似性

    Contains 1 DAGKc domain.
  • Information by UniProt

  • 参照データベース

    • 別名

      • SK 1 antibody
      • SK1 antibody
      • Sphingosine kinase 1 antibody
      • SPHK 1 antibody
      • SPHK antibody
      • Sphk1 antibody
      • SPHK1_HUMAN antibody
      • SPK antibody
      • SPK 1 antibody
      see all

    画像

    • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      All lanes : Anti-SPHK1 antibody [EPR24511-37] (ab302714) at 1/1000 dilution

      Lane 1 : Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
      Lane 2 : Hela transfected with siRNA specifically targeting SPHK1, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 43 kDa
      Observed band size: 42-52 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302714, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      he additional band at ~50 kDa is expected to be isoform 2 (Q9NYA1-2) of SPHK1 and its expression is also significantly reduced through SPHK1 knockdown.

    • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      All lanes : Anti-SPHK1 antibody [EPR24511-37] (ab302714) at 1/1000 dilution

      Lane 1 : Human kidney tissue lysate
      Lane 2 : Human heart tissue lysate
      Lane 3 : Human lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

      Predicted band size: 43 kDa
      Observed band size: 42-52 kDa why is the actual band size different from the predicted?



      This data was developed using ab302714, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      Exposure time: Lane 1 and 2: 180 seconds, Lane 3: 15 seconds

    • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      All lanes : Anti-SPHK1 antibody [EPR24511-37] (ab302714) at 1/1000 dilution

      Lane 1 : U-118 MG (human brain glioma cell), whole cell lysate
      Lane 2 : SW1353 (human chondrosarcoma fibroblast), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 43 kDa
      Observed band size: 42-52 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds
    • Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Western blot - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      All lanes : Anti-SPHK1 antibody [EPR24511-37] (ab302714) at 1/1000 dilution

      Lane 1 : Hela (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 43 kDa
      Observed band size: 42-52 kDa why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      This data was developed using ab302714, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

    • Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

      This data was developed using ab302714, the same antibody clone in a different buffer formulation.

      SPHK1 was immunoprecipitated from 0.35 mg U-118 MG (human brain glioblastoma cell) whole cell lysate with ab302714 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302714 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: U-118 MG (human brain glioblastoma cell) whole cell lysate 10ug

      Lane 2: ab302714 IP in U-118 MG whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302714 in U-118 MG whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds

       Observed MW (kDa): 42-52

    • Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Immunoprecipitation - Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

      This data was developed using ab302714, the same antibody clone in a different buffer formulation.

      SPHK1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab302714 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302714 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

      Lane 2: ab302714 IP in HepG2 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302714 in HepG2 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds

      Observed MW (kDa): 42-52

    • Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)
      Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free) (AB302715)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302715 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302715 は論文での使用が確認できていません。

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