AB233646

Anti-SPG3A/ATL1 抗体 [1F6B12]

Name: Anti-SPG3A/ATL1 抗体 [1F6B12] (ab233646) | Abcam
Brand: Abcam Limited
SKU: AB233646
Price: 74000 JPY
Availability: InStock

Anti-SPG3A/ATL1 antibody [1F6B12]

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(2 Publications)

Mouse Monoclonal SPG3A/ATL1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATL1 aa 1-100.

別名を表示する

GBP3, SPG3A, ATL1, Atlastin-1, Brain-specific GTP-binding protein, GTP-binding protein 3, Guanine nucleotide-binding protein 3, Spastic paraplegia 3 protein A, GBP-3, hGBP3

2 Images

Supplier Data

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Expected MW is 41kDa.

All lanes:

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution

All lanes:

Recombinant human SPG3A/ATL1 protein.

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

All lanes:

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

SPG3A/ATL1 (aa 1-100)-hlgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 64 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

1F6B12

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human ATL1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8WXF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified from tissue culture supernatant.

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SPG3A also known as Atlastin-1 or ATL1 is a GTPase enzyme with a molecular mass of approximately 64 kDa. This protein is highly important in the mechanical process of membrane trafficking and the maintenance of the endoplasmic reticulum (ER) network. Atlastin-1 is expressed predominantly in neurons especially in the cerebral cortex and the hippocampus where it plays a role in neural function.

Biological function summary

Atlastin-1 is involved in the fusion of ER membranes contributing to the network's tubular structure. The protein facilitates the dimerization and fusion of ER membranes a function that is fundamental for creating a continuous and smooth ER network. While it does not form part of a large protein complex its role in membrane dynamics involves interaction with other proteins that maintain ER structure and function.

Pathways

Atlastin-1 participates in the ER and Golgi-related pathways essential for protein trafficking and cellular homeostasis. It has significant interactions in these pathways with proteins like REEP1 and spastin both of which assist in maintaining the dynamic structure of the ER. These interactions underline Atlastin-1's contribution to vesicle transport and membrane fusion processes.

Mutations in the Atlastin-1 gene can lead to hereditary spastic paraplegia (HSP) a group of inherited disorders characterized by weakness and stiffness of the legs. This protein interacts in the context of HSP with proteins like spastin both of which are implicated in similar neuronal membrane dynamics disorders. Another condition linked to Atlastin-1 dysregulation is neuropathy where the protein's failure to maintain ER integrity can contribute to neuronal degradation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis (PubMed : 27619977). May also regulate Golgi biogenesis. May regulate axonal development.

See full target information ATL1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23237 PubMed36193555

2022

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guanli Zhang,Yan Feng,Min Wang,Xin Liu

Cancer immunology, immunotherapy : CII 71:1075-1091 PubMed34562123

2021

LncRNA MIR155HG induces M2 macrophage polarization and drug resistance of colorectal cancer cells by regulating ANXA2.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhou,Jian Li,Mingmei Liao,Qi Zhang,Mei Yang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPG3A/ATL1 抗体 [1F6B12]

Anti-SPG3A/ATL1 antibody [1F6B12]

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Western blot - Anti-SPG3A/ATL1 antibody [1F6B12] (AB233646)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise