Anti-Spermidine synthase 抗体 (ab111884)
Key features and details
- Rabbit polyclonal to Spermidine synthase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Spermidine synthase antibody
Spermidine synthase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Spermidine synthase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 22-209 of Human Spermidine synthase (AAH00309).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab111884 is purified by antigen-affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111884の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0).
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| 特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0). |
ターゲット情報
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機能
Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine. -
パスウェイ
Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. -
配列類似性
Belongs to the spermidine/spermine synthase family.
Contains 1 PABS (polyamine biosynthesis) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 6723 Human
- Omim: 182891 Human
- SwissProt: P19623 Human
- Unigene: 76244 Human
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別名
- OTTHUMP00000002170 antibody
- PAPT antibody
- Putrescine aminopropyltransferase antibody
see all
画像
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Western blot - Anti-Spermidine synthase antibody (ab111884)Anti-Spermidine synthase antibody (ab111884) at 1/5000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spermidine synthase antibody (ab111884)ab111884 at 1/500 dilution staining Spermidine synthase in Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab111884 は 2 報の論文で使用されています。
- Chen J et al. Supramolecular trap for catching polyamines in cells as an anti-tumor strategy. Nat Commun 10:3546 (2019). PubMed: 31391464
- Bistulfi G et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . PubMed: 26910893