Anti-Spermidine 抗体 (ab7318)
Key features and details
- Rabbit polyclonal to Spermidine
- Suitable for: IHC-P
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Spermidine antibody -
製品の詳細
Rabbit polyclonal to Spermidine -
由来種
Rabbit -
特異性
Reacts with spermidine and cross reacts with spermine. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Spermidine conjugated to KLH.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Spermidine is a polyamine compound was originally isolated from semen. It is found ribosomes and living tissues and has various metabolic functions.
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spermidine antibody (ab7318)Image from Chung-Ping liao et. al. J. Biol. Chem., Mar 2009; 284: 8174 - 8184. (Fig 3B).
ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab7318 は 7 報の論文で使用されています。
- Ríos DS et al. Changes in the Localization of Polyamine Spermidine in the Rat Retina with Age. Biomedicines 11:N/A (2023). PubMed: 37189626
- Aihara S et al. Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis. Commun Biol 6:676 (2023). PubMed: 37380734
- Al-Habsi M et al. Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice. Science 378:eabj3510 (2022). PubMed: 36302005
- Bian X et al. AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation. Clin Transl Med 11:e352 (2021). PubMed: 33783988
- Yang K et al. Measuring non-polyaminated lipocalin-2 for cardiometabolic risk assessment. ESC Heart Fail 4:563-575 (2017). PubMed: 29154418
- Rossi G et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. Biomed Res Int 2015:172756 (2015). IHC-P . PubMed: 26550563
- Liao CP et al. Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages. J Biol Chem 284:8174-84 (2009). IHC-P ; Rat . PubMed: 19158080