Anti-SPEF1 抗体 (ab151100)
Key features and details
- Rabbit polyclonal to SPEF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPEF1 antibody -
製品の詳細
Rabbit polyclonal to SPEF1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to amino acids 31-151 of Human SPEF1 (UniProt Q9Y4P9-2).
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特記事項
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151100の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/250 - 1/500. Predicted molecular weight: 27 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/250 - 1/500. Predicted molecular weight: 27 kDa. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia. -
参照データベース
- Entrez Gene: 25876 Human
- Omim: 610674 Human
- SwissProt: Q9Y4P9 Human
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別名
- C20orf28 antibody
- Calponin homology and microtubule associated protein antibody
- CLAMP antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPEF1 antibody (ab151100)Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SPEF1 with ab151100 at 1/50 dilution.
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Western blot - Anti-SPEF1 antibody (ab151100)All lanes : Anti-SPEF1 antibody (ab151100) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 27 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab151100 は論文での使用が確認できていません。