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リコンビナントRabMAb

Anti-Spata18 抗体 [EPR13704] (ab180154)

Submit a review Submit a question References (3)
Western blot - Anti-Spata18 antibody [EPR13704] (ab180154)
  • Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (ab180154)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (ab180154)
  • Anti-Spata18 antibody [EPR13704] (ab180154)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13704] to Spata18
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Spata18 antibody [EPR13704]
    Spata18 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13704] to Spata18

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human Spata18 aa 100-250. The exact sequence is proprietary.
    Database link: Q8TC71

  • ポジティブ･コントロール

    • HepG2, HeLa, LnCaP and HT1080 cell lysates; Human kidney tissue; HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13704

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180154の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
特記事項
ICC/IF
1/500.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

ターゲット情報

画像

  • Western blot - Anti-Spata18 antibody [EPR13704] (ab180154)
    Western blot - Anti-Spata18 antibody [EPR13704] (ab180154)
    All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : HT1080 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (ab180154)
    Immunocytochemistry/ Immunofluorescence - Anti-Spata18 antibody [EPR13704] (ab180154)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (ab180154)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spata18 antibody [EPR13704] (ab180154)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Spata18 antibody [EPR13704] (ab180154)
    Anti-Spata18 antibody [EPR13704] (ab180154)

参考文献 (3)

ab180154 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180154 は 3 報の論文で使用されています。

  • Hamada K  et al. MIEAP and ATG5 are tumor suppressors in a mouse model of BRAFV600E-positive thyroid cancer. Front Endocrinol (Lausanne) 13:932754 (2022). PubMed: 36187114
  • Song WH  et al. Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy. Cells 10:N/A (2021). PubMed: 34572103
  • Ren M  et al. Extramitochondrial cardiolipin suggests a novel function of mitochondria in spermatogenesis. J Cell Biol 218:1491-1502 (2019). PubMed: 30914420

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