Anti-SPANX 抗体 (ab47252)
Key features and details
- Rabbit polyclonal to SPANX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SPANX antibody
SPANX 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SPANX -
由来種
Rabbit -
特異性
The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
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免疫原
Synthetic peptide:
GDSDPQPAPKKMKTSES
, corresponding to internal sequence amino acids 31-47 of Human SPANX C -
ポジティブ･コントロール
- Nuclear extract from VWM105 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 11 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 11 kDa. |
ターゲット情報
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組織特異性
Detected in testis, melanoma and bladder carcinoma. -
配列類似性
Belongs to the SPAN-X family. -
発生段階
Detected in round and elongating spermatids. -
細胞内局在
Cytoplasm. Nucleus. Associated with nuclear craters. - Information by UniProt
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参照データベース
- Entrez Gene: 64663 Human
- Omim: 300330 Human
- SwissProt: A0FGV1 Human
- SwissProt: A0FGV4 Human
- SwissProt: A0FGW6 Human
- SwissProt: A0FGX0 Human
- SwissProt: Q32WM2 Human
- SwissProt: Q9NY87 Human
see all -
別名
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
see all
画像
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Western blot - Anti-SPANX antibody (ab47252)All lanes : Anti-SPANX antibody (ab47252) at 1/1000 dilution
Lane 1 : Nuclear extract from VWM105 cells
Lane 2 : purified recombinant
SPANX-C fusion protein.
Lane 3 : purified
recombinant SPANX-B, protein.
Lane 4 : purified
recombinant SPANX-C, protein.
Lane 5 : purified
recombinant SPANX-N, protein.
Secondary
Lanes 1-2 : IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab47252 は 1 報の論文で使用されています。
- Lazar I et al. SPANX Control of Lamin A/C Modulates Nuclear Architecture and Promotes Melanoma Growth. Mol Cancer Res 18:1560-1573 (2020). PubMed: 32571981
