AB47252

Anti-SPANX 抗体

Anti-SPANX antibody

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(1 Publication)

Rabbit Polyclonal SPANX antibody. Suitable for WB and reacts with Recombinant full length protein, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPANXC aa 1-50.

別名を表示する

Sperm protein associated with the nucleus on the X chromosome C, Cancer/testis antigen 11.3, Cancer/testis-associated protein CTp11, Nuclear-associated protein SPAN-Xc, SPANX family member C, CT11.3, SPANX-C, SPANXC

1 Images

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Western blot - Anti-SPANX antibody (AB47252)

All lanes:

Western blot - Anti-SPANX antibody (ab47252) at 1/1000 dilution

Lane 1:

Nuclear extract from VWM105 cells

Lane 2:

purified recombinantSPANX-C fusion protein

Lane 3:

purifiedrecombinant SPANX-B, protein

Lane 4:

purifiedrecombinant SPANX-C, protein

Lane 5:

purifiedrecombinant SPANX-N, protein

Secondary

All lanes:

IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122)

Predicted band size: 11 kDa

Observed band size: 17 kDa,60 kDa,70 kDa,80 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human SPANXC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NY87

特異性

The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SPANX also referred to as Sperm Protein Associated with the Nucleus on the X chromosome is a protein involved in cellular mechanisms. This protein has an approximate mass of 12-14 kDa. Expression of SPANX occurs mainly in the testis and it localizes primarily to nuclear compartments in mature spermatozoa. The SPANX family of proteins includes several paralogs each with specific but similar roles within the reproductive system. It seems the structural features of SPANX such as its nuclear localization signals play a role in its capability to bind chromatin and influence nuclear events.

Biological function summary

SPANX proteins participate in spermatogenesis contributing to the process of sperm maturation. These proteins lack known enzymatic activities but associate with chromatin to support sperm packaging and genome stability. Evidence does not suggest that SPANX forms part of a larger complex but its functions enable critical chromatin condensation needed for proper sperm morphogenesis. By maintaining genome stability during sperm formation SPANX influences reproductive success.

Pathways

Proteins like SPANX support pathways related to chromatin organization and spermatogenesis. In spermatogenesis SPANX proteins work alongside transition proteins and protamines in facilitating chromatin remodeling. The role of SPANX intersects with pathways of chromatin restructuring notably linked to histones and DNA repair proteins. While it does not interact directly with well-characterized signaling pathways SPANX remains essential in localized chromatin pathway interactions during sperm cell development.

Proteins from the SPANX family show expression in certain cancers including melanoma and prostate cancer. These disorders often exhibit dysregulated chromatin dynamics where SPANX expression alters cellular chromatin states. Investigations link overexpression of SPANX to tumorigenicity likely due to its effects on chromatin structures and subsequent gene expression patterns. Additionally SPANX associates indirectly with proteins like MAGE-A that also modulate cellular growth and tumor-related pathways suggesting overlapping roles in cancer progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information SPANXC

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 18:1560-1573 PubMed32571981

2020

SPANX Control of Lamin A/C Modulates Nuclear Architecture and Promotes Melanoma Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ikrame Lazar,Bertrand Fabre,Yongmei Feng,Ali Khateb,Patrick Turko,Julia M Martinez Gomez,Dennie T Frederick,Mitchell P Levesque,Lea Feld,Gao Zhang,Tongwu Zhang,Brian James,Jeny Shklover,Emily Avitan-Hersh,Ido Livneh,Marzia Scortegagna,Kevin Brown,Ola Larsson,Ivan Topisirovic,Haguy Wolfenson,Meenhard Herlyn,Keith Flaherty,Reinhard Dummer,Ze'ev A Ronai

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPANX 抗体

Anti-SPANX antibody

Western blot - Anti-SPANX antibody (AB47252)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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