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リコンビナントRabMAb

Anti-Sp8 抗体 [EPR26224-86] - BSA and Azide free (ab302916)

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Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
  • Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
  • Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26224-86] to Sp8 - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free
    Sp8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26224-86] to Sp8 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details
    適用なし: Flow Cyt (Intra) or ICC/IF

  • 種交差性

    交差種: Rat, Human
    非交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat olfactory bulb tissue lysate. IHC-P: Human prostate and rat brain tissue. HEK-293T cells transfected with a human SP8 expression vector containing a myc-His tag cell pellet. IHC-Fr: Rat olfactory bulb (fresh) frozen tissue.

  • 特記事項

    AB302915 does not react with human in WB, ICC/IF or Flow Cytometry (Intra); mouse in WB, IHC or IHC-Fr.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302916の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30-60 kDa (predicted molecular weight: 48 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 30-60 kDa (predicted molecular weight: 48 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or ICC/IF.

ターゲット情報

  • 機能

    Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.

  • 配列類似性

    Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BTD antibody
      • Buttonhead antibody
      • Homolog of Btd antibody
      • OTTHUMP00000120017 antibody
      • Sp 8 antibody
      • SP8 antibody
      • Sp8 transcription factor antibody
      • SP8_HUMAN antibody
      • Specificity protein 8 antibody
      • Trans acting transcription factor 8 antibody
      • Transcription factor Sp8 antibody
      see all

    画像

    • Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
      Western blot - Anti-Sp8 antibody [EPR26224-86] - BSA and Azide free (ab302916)
      All lanes : Anti-Sp8 antibody [EPR26224-86] (ab302915) at 1/1000 dilution

      Lane 1 : Rat olfactory bulb tissue lysate
      Lane 2 : Rat spleen tissue lysate
      Lane 3 : Rat lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 48 kDa
      Observed band size: 30-60 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using AB302915, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST

      Low expression: spleen, lung (consistent with the predicted expression pattern).

      Bands below 60 kDa may due to degradation.

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

      This data was developed using AB302915, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling Sp8 with ab302915 at 1/50 (10.6 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on human prostate. The section was incubated with ab302915 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

      This data was developed using AB302915, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling Sp8 with ab302915 at 1/200 dilution (2.65 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). (Panel A) Nuclear staining on olfactory bulb of rat brain at low-powered (magnification, x10) and (Panel B) nuclear staining on rat olfactory bulb at high-powered (magnification, x400). The section was incubated with ab302915 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

      This data was developed using AB302915, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells labeling Sp8 with ab302915 at 1/5000 dilution (0.106 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection). Nuclear staining on HEK-293T cells transfected with a human SP8 expression vector containing a myc-His tag cell pallet (Panel A). No staining on HEK-293T cells transfected with empty vector containing a his tag cell pellet (Panel B). The section was incubated with ab302915 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use Leica DS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
      Immunohistochemistry (Frozen sections) - Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

      This data was developed using AB302915, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat olfactory bulb (fresh) tissue labeling Sp8 with ab302915 at 1/500 (1.06 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat olfactory bulb. The nuclear counterstain was DAPI (Blue). The section was incubated with ab302915 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.

    • Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)
      Anti-Sp8 antibody [EPR26224-86] (BSA and Azide free) (AB302916)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302916 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302916 は論文での使用が確認できていません。

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