AB22552

Anti-Sp7 / Osterix 抗体

Name: Anti-Sp7 / Osterix 抗体 (ab22552) | Abcam
Brand: Abcam Limited
SKU: AB22552
Price: 65000 JPY
Availability: InStock
Rating: 5 (24 reviews)

Anti-Sp7 / Osterix antibody

BOND RX™ Validated
詳細を見る

(24 Reviews)

(504 Publications)

Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody detecting Sp7 / Osterix in IHC-P. Suitable for Mouse, Rat.

- Over 420 publications
- Trusted since 2006

別名を表示する

Osx, Sp7, Transcription factor Sp7, C22, Zinc finger protein osterix

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal rat E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.

This image is courtesy of an anonymous collaborator

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.

IHC-P

AbReview6355****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

This image is courtesy of an anonymous Abreview

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal mouse E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

CiteAb

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry-immunofluorescence using Anti-Sp7 / Osterix antibody, ab22552. Publication image from Wang, L. et al., 2017, Nat Commun, 29101351. Legend direct from paper.

Deletion of Ezh2 in nestin+ cells during early puberty impairs osteogenesis. a Schematic diagram indicating the experimental workflow in Nestin-CreERT2 : : Ezh2flox/flox (Ezh2 iKO) mice. In short, three-week-old male Ezh2 iKO mice and Nestin-CreERT2 mice (WT) were injected with three doses of tamoxifen (100 mg/kg B.W., every other day. The mice were humanely killed at 4 weeks of age. Double-immunofluorescence staining of femoral metaphysis sections was performed using antibodies against nestin (green) and Ezh2 (red) b. Quantification of the percentage of nestin+ cells that express Ezh2 in primary spongiosa (c). Double-immunofluorescence staining of femur sections was performed using antibodies against CD31 (green) and endomucin (Emcn) (red) (d). Quantitative analysis of relative fluorescence intensities in primary spongiosa (e). Immunofluorescence staining of femur sections using antibodies against osterix (Osx) (f) and osteocalcin (Ocn) (h). DAPI stains nuclei blue. Quantitative analysis of Osx+ and Ocn+ cells in primary spongiosa is shown in g, i, respectively. Representative images of trichrome staining of the metaphyseal trabecular bone (j). Five mice per group. Data are represented as mean ± s.e.m. *P < 0.05 as determined by Student’s t-tests

CiteAb

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry using Anti-Sp7 / Osterix antibody, ab22552. Publication image from Sheu, T. J. et al., 2016, Nat Commun, 26830436. Legend direct from paper.

SuSCs improve bone healing through direct engraftment.The reparative ability of SuSC is examined by implanting without (a,d,h–j), or with suture mesenchymal cells containing Axin2– (b,e) or Axin2+ (c,f,k–s) in the injury repair model. Two (a–c,h–s) and 4 (d–f) weeks after operation the healing process is assessed by micro CT (a–f), β-gal staining (h–m,o–p,r–s) and immunostaining of Osx (n,p) and Sost (q,s) in whole mounts (a–f,h,k) and sections (i–j,l–s). The graph shows the average per cent of healing at 2 and 4 weeks post operation (g, n=3, mean±s.e.m.; P value<0.05, Student t-test). Arrows, arrowheads and bracket indicate osteoprogenitors, osteocytes and unhealed area, respectively. Images are representatives of three independent experiments. Scale bar, 500 µm (a–f). Scale bar, 1 mm (h,k). Scale bar, 200 µm (i,l). Scale bar, 50 µm (j,m–s).

CiteAb

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry using Anti-Sp7 / Osterix antibody, ab22552. Publication image from Sheu, T. J. et al., 2016, Nat Commun, 26830436. Legend direct from paper.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." } } }

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製品の詳細

Product Specifications
Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody and is validated for use in IHC-P in mouse, rat samples.
Anti-Sp7 / Osterix antibody (ab22552) specifically detects Sp7 / Osterix (UniProt ID: Q8TDD2; Molecular weight: 45kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Sp7 / Osterix antibody (ab22552) has high sensitivity and specificity.
Anti-Sp7 / Osterix antibody (ab22552) has been cited over 422 times in peer reviewed journals and is trusted by the scientific community.
Anti-Sp7 / Osterix antibody (ab22552) has 23 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional activator essential for osteoblast differentiation (PubMed : 11792318, PubMed : 17510056, PubMed : 30026585). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (PubMed : 11792318, PubMed : 17510056).

See full target information Sp7

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文献 (504)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:35547 PubMed41073566

2025

Total flavonoids of rhizoma drynariae improved the osteogenic and pro-angiogenic capacities of BMSCs to accelerate long bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hengjun Huang,Silu Li,Huang Zhan,Fenfang Gong,Jian Liu,Zhenya Liu,Hui Li,Chengyu Yang

International journal of oral science 17:65 PubMed40998797

2025

PLAGL1-IGF2 axis regulates osteogenesis of postnatal condyle development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrui Sun,Jingyi Xu,Yue Xu,Yili Liu,Enhui Yao,Jiahui Du,Xinquan Jiang

Communications biology 8:1309 PubMed40883591

2025

Ovalbumin-induced asthma leads to bone loss with Piezo channel suppression in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqi Gao,Takeshi Sawada,Ailin Cao,Reiko U Yoshimoto,Yu Yamaguchi,Yukie Takahashi,Takaichi Fukuda,Yasuyoshi Ohsaki,Reona Aijima,Tomoko Kadowaki,Takayuki Tsukuba,Mizuho A Kido

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Frontiers in bioengineering and biotechnology 13:1639117 PubMed40837020

2025

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Hai-Jing Zhang,Hui Wang,Gang Ji,Ya-Heng Zhao,Gao-Cen Li,Shao-Shi Guo,Lu-Feng Lin,Yu-Jie Jin,Xue-Li Zhang,Xin-Yu Nan,Chang-Cheng Liu,Guo-Bin Liu

Frontiers in endocrinology 16:1570685 PubMed40687591

2025

Jinkui Shenqi Pill accelerates osteoporotic fracture healing by promoting bone formation through neurosensory PGE2/EP4/p-CREB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihao Xi,Danqing Fu,Dan Xu,Ruhan Shen,Yan Zhao,Haoqiang Dai,Chenjie Xia,Peihong Zhou

Nature communications 16:5425 PubMed40593719

2025

Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Liu,Lu Xing,Wenlong Huang,Ning Ji,Hang Zhao,Qianming Chen,Xianglong Han,Ding Bai,Xuefeng Zhao

Organogenesis 21:2519649 PubMed40570323

2025

SS-31 Targets NOS2 to Enhance Osteogenic Differentiation in Aged BMSCs by Restoring Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Duan,Qindong Zhang,Jinqiang Zhu,Jiaming Wang

Journal of orthopaedic translation 52:387-403 PubMed40476065

2025

Vitamins D and K jointly protect against osteoarthritis via regulating OSCAR during osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Qianhua Ou,Hong Huang,Delong Li,Jianmao Chen,Song Xue,Zuoqing Zhou,Guangfeng Ruan,Changhai Ding

Nature communications 16:4330 PubMed40346043

2025

Self-propagating wave drives morphogenesis of skull bones in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yiteng Dang,Johanna Lattner,Adrian A Lahola-Chomiak,Diana Alves Afonso,Elke Ulbricht,Anna Taubenberger,Steffen Rulands,Jacqueline M Tabler

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sp7 / Osterix 抗体

Anti-Sp7 / Osterix antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry - Anti-Sp7 / Osterix antibody (AB22552)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (504)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise