AB308364

Anti-SP1 抗体 [HL1396]

Anti-SP1 antibody [HL1396]

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.

別名を表示する

TSFP1, SP1, Transcription factor Sp1

6 Images

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SP1 protein using ab308364 at 1/500 dilution (green). Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of rat intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

HL1396

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Mouse, Rat

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information.

P08047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).

See full target information SP1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:737 PubMed40254586

2025

Targeting GSK3β and signaling pathways in breast cancer: role of individual members of miR- 23/24/27 cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Harshi Gupta,Anushka Raghubansi, Bharat,Kritika Sharma,Krittika Zutshi,Partibha Panchal,Sushant Bhattacharya,Piyush Ranjan,Gopal Puri,Neeru Saini

Folia biologica 70:219-228 PubMed39692576

2024

Effects of Different Light Environments with Varying Spectral Composition on the Axial Lengths and Scleral Specificity Protein 1 and Collagen Type I Expression in Juvenile Guinea Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbao Yuan,Yuliang Wang,Xinyu Xu,Mei Yang,Yipeng Fan,Xiaopan Shi,Lulu Sun,Mingyu Shan,Lei Ma

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SP1 抗体 [HL1396]

Anti-SP1 antibody [HL1396]

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (2)

Abcam product promise