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リコンビナントRabMAb

Anti-SOX9 抗体 [EPR12755-50] - C-terminal (ab184547)

Submit a review Submit a question References (3)
Western blot - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
  • Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
  • Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SOX9 antibody [EPR12755-50] - C-terminal
    SOX9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12755-50] to SOX9 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2, SW480 and Caco2 cell lysates; Caco2 and HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184547の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
特記事項
Flow Cyt (Intra)
1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/500.
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

  • 機能

    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

  • 関連疾患

    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.

  • 配列類似性

    Contains 1 HMG box DNA-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • campomelic dysplasia autosomal sex reversal antibody
      • CMD 1 antibody
      • CMD1 antibody
      • CMPD 1 antibody
      • CMPD1 antibody
      • SOX 9 antibody
      • Sox9 antibody
      • SOX9_HUMAN antibody
      • SRA 1 antibody
      • SRA1 antibody
      • SRXX2 antibody
      • SRXY10 antibody
      • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
      • SRY (sex determining region Y) box 9 antibody
      • SRY (sex determining region Y)-box 9 antibody
      • SRY (sex-determining region Y)-box 9 protein antibody
      • SRY related HMG box gene 9 antibody
      • Transcription factor SOX 9 antibody
      • Transcription factor SOX-9 antibody
      • transcription factor SOX9 antibody
      see all

    画像

    • Western blot - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      Western blot - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      All lanes : Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 56 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed Caco2 cells, labeling SOX9 with ab184547 at 1/500 dilution (red). Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

      Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells, labeling SOX9 with ab184547 at 1/500 dilution (green). Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

    • Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

      Intracellular flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed)labeling SOX9 with ab184547 at 1/200 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. 

       

       

    • Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)
      Anti-SOX9 antibody [EPR12755-50] - C-terminal (ab184547)

    参考文献 (3)

    ab184547 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184547 は 3 報の論文で使用されています。

    • Domingo-Muelas A  et al. Post-transcriptional control of a stemness signature by RNA-binding protein MEX3A regulates murine adult neurogenesis. Nat Commun 14:373 (2023). PubMed: 36690670
    • Fabra-Beser J  et al. Differential Expression Levels of Sox9 in Early Neocortical Radial Glial Cells Regulate the Decision between Stem Cell Maintenance and Differentiation. J Neurosci 41:6969-6986 (2021). PubMed: 34266896
    • Balogh E  et al. Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner. Arterioscler Thromb Vasc Biol 39:1088-1099 (2019). PubMed: 31070451

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