Anti-SOX8 抗体 (ab27655)
Key features and details
- Rabbit polyclonal to SOX8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Chicken
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SOX8 antibody
SOX8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SOX8 -
由来種
Rabbit -
特異性
Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9 -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Chicken -
免疫原
Synthetic peptide corresponding to Chicken SOX8 aa 400-500 (C terminal).
Database link: P57074 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27655の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
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機能
May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 395483 Chicken
- Entrez Gene: 20681 Mouse
- SwissProt: P57074 Chicken
- SwissProt: Q04886 Mouse
- Unigene: 258220 Mouse
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別名
- MGC24837 antibody
- SOX 8 antibody
- Sox8 antibody
see all
画像
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Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
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All lanes : Anti-SOX8 antibody (ab27655) at 1/500 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab27655 は 19 報の論文で使用されています。
- Mac Donald K & Iulianella A The actin-cytoskeleton associating protein BASP1 regulates neural progenitor localization in the neural tube. Genesis 60:e23464 (2022). PubMed: 34897971
- Inubushi T et al. The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival. PLoS Genet 18:e1009765 (2022). PubMed: 35839257
- Wang Y et al. SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice. Cell Rep 41:111842 (2022). PubMed: 36543123
- Wang Y et al. PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination. Cell Rep 37:109695 (2021). PubMed: 34610310
- Zhang S et al. HIFa Regulates Developmental Myelination Independent of Autocrine Wnt Signaling. J Neurosci 41:251-268 (2021). PubMed: 33208471