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リコンビナントRabMAb

Anti-SOX6 抗体 [EPR23564-282] (ab245215)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
  • Anti-SOX6 antibody [EPR23564-282] (ab245215)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23564-282] to SOX6
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SOX6 antibody [EPR23564-282]
    SOX6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23564-282] to SOX6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ChIP,Flow Cyt,ICC,ICC/IF,IHC-Fr,IP or WB

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human astrocytoma and skin tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245215の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ChIP,Flow Cyt,ICC,ICC/IF,IHC-Fr,IP or WB.

ターゲット情報

  • 機能

    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.

  • 組織特異性

    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.

  • 配列類似性

    Contains 1 HMG box DNA-binding domain.

  • 翻訳後修飾

    Sumoylation inhibits the transcriptional activity.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • HSSOX 6 antibody
      • HSSOX6 antibody
      • SOX 6 antibody
      • sox6 antibody
      • SOX6_HUMAN antibody
      • SOXD antibody
      • SRY (sex determining region Y) box 6 antibody
      • SRY box 6 antibody
      • SRY box containing gene 6 antibody
      • Transcription factor SOX 6 antibody
      • Transcription factor Sox-6 antibody
      • Transcription factor SOX6 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)

      Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human astrocytoma (PMID: 14691456, PMID: 15696972). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)

      Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in basal cells of human skin (PMID: 32088305). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX6 antibody [EPR23564-282] (ab245215)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SOX6 with ab245215 at 1/500 (0.892 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human spleen (PMID: 14691456). The section was incubated with ab245215 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-SOX6 antibody [EPR23564-282] (ab245215)
      Anti-SOX6 antibody [EPR23564-282] (ab245215)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab245215 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245215 は 2 報の論文で使用されています。

    • Xu Y & Zhan X lncRNA KCNQ1OT1 regulated high glucose-induced proliferation, oxidative stress, extracellular matrix accumulation, and inflammation by miR-147a/SOX6 in diabetic nephropathy (DN). Endocr J 69:511-522 (2022). PubMed: 34911869
    • Chen YX  et al. LncRNA Dlx6os1 Accelerates Diabetic Nephropathy Progression by Epigenetically Repressing SOX6 via Recruiting EZH2. Kidney Blood Press Res 47:177-184 (2022). PubMed: 35038705

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