Anti-SOX2 抗体 [9-9-3] (ab79351)
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-22-anti-sox2-993-antibody-immunocytochemistry-mes-embryonic-stem-cell-mouse.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)")
Key features and details
- Mouse monoclonal [9-9-3] to SOX2
- Suitable for: ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Human, Apteronotus leptorhynchus
- Isotype: IgG1
製品の概要
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製品名
Anti-SOX2 antibody [9-9-3]
SOX2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9-9-3] to SOX2 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human, Apteronotus leptorhynchus
交差が予測される動物種: Rat, Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human SOX2 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab80398) -
ポジティブ･コントロール
- ICC/IF: NCCIT, mES and F9 cells. WB: F9 and PC-3 whole cell lysate. Flow Cyt (Intra): F9 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 6.97% L-Arginine, PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
9-9-3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (4) |
1/200.
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| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 34 kDa).
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| Flow Cyt (Intra) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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ICC/IF
1/200. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 34 kDa). |
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Flow Cyt (Intra)
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
関連疾患
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
配列類似性
Contains 1 HMG box DNA-binding domain. -
翻訳後修飾
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 396105 Chicken
- Entrez Gene: 784383 Cow
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 407739 Pig
- Entrez Gene: 499593 Rat
- Entrez Gene: 398000 Xenopus laevis
- Omim: 184429 Human
see all -
別名
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)
ab79351 staining SOX2 in mES cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab79351 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-258306-anti-sox2-antibody-9-9-3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on NCCIT cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
-ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution. -
![Western blot - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-258054-anti-sox2-antibody-9-9-3-western-blot.jpg)
Western blot - Anti-SOX2 antibody [9-9-3] (ab79351)All lanes : Anti-SOX2 antibody [9-9-3] (ab79351) at 1/10000 dilution
Lane 1 : NCCIT whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-94890-anti-sox2-antibody-9-9-3-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SOX2 antibody [9-9-3] (ab79351)Overlay histogram showing F9 cells stained with ab79351 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79351, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed F9 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-258310-anti-sox2-antibody-9-9-3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on F9 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
-ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-94891-anti-sox2-antibody-9-9-3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)This image is courtesy of an abreview by Joe Segal.ICC/IF image of ab79351 stained D3 mouse embryonic stem cells. The cells were fixed in 4% Paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT, before incubation with ab79351 at a 1/100 dilution for 2 hours at RT. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse polyclonal, used at 1/200 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)](https://www.abcam.co.jp/ps/products/79/ab79351/Images/ab79351-258314-anti-sox2-antibody-9-9-3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [9-9-3] (ab79351)Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) (Negative control) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing no staining on NIH/3T3 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
The negative controls are as follows:
-ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
-ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (101)
ab79351 は 101 報の論文で使用されています。
- Xia KS et al. An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration. Bioact Mater 21:69-85 (2023). PubMed: 36017070
- Wang X et al. SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses. MedComm (2020) 4:e198 (2023). PubMed: 36582303
- Yue T et al. Hemoglobin Derived from Subarachnoid Hemorrhage-Induced Pyroptosis of Neural Stem Cells via ROS/NLRP3/GSDMD Pathway. Oxid Med Cell Longev 2023:4383332 (2023). PubMed: 36703912
- Xu M et al. L-arginine homeostasis governs adult neural stem cell activation by modulating energy metabolism in vivo. EMBO J 42:e112647 (2023). PubMed: 36740997
- Cruz EM et al. A Gelatin Methacrylate-Based Hydrogel as a Potential Bioink for 3D Bioprinting and Neuronal Differentiation. Pharmaceutics 15:N/A (2023). PubMed: 36839949